The lactoperoxidase system links anion transport to host defense in cystic fibrosis

Gregory E Conner, Corinne Wijkstrom-Frei, Scott H. Randell, Vania E. Fernandez, Matthias A Salathe

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Chronic respiratory infections in cystic fibrosis result from CFTR channel mutations but how these impair antibacterial defense is less clear. Airway host defense depends on lactoperoxidase (LPO) that requires thiocyanate (SCN-) to function and epithelia use CFTR to concentrate SCN- at the apical surface. To test whether CFTR mutations result in impaired LPO-mediated host defense, CF epithelial SCN- transport was measured. CF epithelia had significantly lower transport rates and did not accumulate SCN- in the apical compartment. The lower CF [SCN-] did not support LPO antibacterial activity. Modeling of airway LPO activity suggested that reduced transport impairs LPO-mediated defense and cannot be compensated by LPO or H2O2 upregulation.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalFEBS Letters
Volume581
Issue number2
DOIs
StatePublished - Jan 23 2007

Fingerprint

Lactoperoxidase
Cystic Fibrosis
Anions
Epithelium
Mutation
Respiratory Tract Infections
Up-Regulation

Keywords

  • CFTR
  • Cystic fibrosis
  • Host defense
  • Hydrogen peroxide
  • Lactoperoxidase
  • Thiocyanate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Conner, G. E., Wijkstrom-Frei, C., Randell, S. H., Fernandez, V. E., & Salathe, M. A. (2007). The lactoperoxidase system links anion transport to host defense in cystic fibrosis. FEBS Letters, 581(2), 271-278. https://doi.org/10.1016/j.febslet.2006.12.025

The lactoperoxidase system links anion transport to host defense in cystic fibrosis. / Conner, Gregory E; Wijkstrom-Frei, Corinne; Randell, Scott H.; Fernandez, Vania E.; Salathe, Matthias A.

In: FEBS Letters, Vol. 581, No. 2, 23.01.2007, p. 271-278.

Research output: Contribution to journalArticle

Conner, GE, Wijkstrom-Frei, C, Randell, SH, Fernandez, VE & Salathe, MA 2007, 'The lactoperoxidase system links anion transport to host defense in cystic fibrosis', FEBS Letters, vol. 581, no. 2, pp. 271-278. https://doi.org/10.1016/j.febslet.2006.12.025
Conner, Gregory E ; Wijkstrom-Frei, Corinne ; Randell, Scott H. ; Fernandez, Vania E. ; Salathe, Matthias A. / The lactoperoxidase system links anion transport to host defense in cystic fibrosis. In: FEBS Letters. 2007 ; Vol. 581, No. 2. pp. 271-278.
@article{a4ec6ec3fcd34282be269017455c570b,
title = "The lactoperoxidase system links anion transport to host defense in cystic fibrosis",
abstract = "Chronic respiratory infections in cystic fibrosis result from CFTR channel mutations but how these impair antibacterial defense is less clear. Airway host defense depends on lactoperoxidase (LPO) that requires thiocyanate (SCN-) to function and epithelia use CFTR to concentrate SCN- at the apical surface. To test whether CFTR mutations result in impaired LPO-mediated host defense, CF epithelial SCN- transport was measured. CF epithelia had significantly lower transport rates and did not accumulate SCN- in the apical compartment. The lower CF [SCN-] did not support LPO antibacterial activity. Modeling of airway LPO activity suggested that reduced transport impairs LPO-mediated defense and cannot be compensated by LPO or H2O2 upregulation.",
keywords = "CFTR, Cystic fibrosis, Host defense, Hydrogen peroxide, Lactoperoxidase, Thiocyanate",
author = "Conner, {Gregory E} and Corinne Wijkstrom-Frei and Randell, {Scott H.} and Fernandez, {Vania E.} and Salathe, {Matthias A}",
year = "2007",
month = "1",
day = "23",
doi = "10.1016/j.febslet.2006.12.025",
language = "English",
volume = "581",
pages = "271--278",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - The lactoperoxidase system links anion transport to host defense in cystic fibrosis

AU - Conner, Gregory E

AU - Wijkstrom-Frei, Corinne

AU - Randell, Scott H.

AU - Fernandez, Vania E.

AU - Salathe, Matthias A

PY - 2007/1/23

Y1 - 2007/1/23

N2 - Chronic respiratory infections in cystic fibrosis result from CFTR channel mutations but how these impair antibacterial defense is less clear. Airway host defense depends on lactoperoxidase (LPO) that requires thiocyanate (SCN-) to function and epithelia use CFTR to concentrate SCN- at the apical surface. To test whether CFTR mutations result in impaired LPO-mediated host defense, CF epithelial SCN- transport was measured. CF epithelia had significantly lower transport rates and did not accumulate SCN- in the apical compartment. The lower CF [SCN-] did not support LPO antibacterial activity. Modeling of airway LPO activity suggested that reduced transport impairs LPO-mediated defense and cannot be compensated by LPO or H2O2 upregulation.

AB - Chronic respiratory infections in cystic fibrosis result from CFTR channel mutations but how these impair antibacterial defense is less clear. Airway host defense depends on lactoperoxidase (LPO) that requires thiocyanate (SCN-) to function and epithelia use CFTR to concentrate SCN- at the apical surface. To test whether CFTR mutations result in impaired LPO-mediated host defense, CF epithelial SCN- transport was measured. CF epithelia had significantly lower transport rates and did not accumulate SCN- in the apical compartment. The lower CF [SCN-] did not support LPO antibacterial activity. Modeling of airway LPO activity suggested that reduced transport impairs LPO-mediated defense and cannot be compensated by LPO or H2O2 upregulation.

KW - CFTR

KW - Cystic fibrosis

KW - Host defense

KW - Hydrogen peroxide

KW - Lactoperoxidase

KW - Thiocyanate

UR - http://www.scopus.com/inward/record.url?scp=33846211109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846211109&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.12.025

DO - 10.1016/j.febslet.2006.12.025

M3 - Article

C2 - 17204267

AN - SCOPUS:33846211109

VL - 581

SP - 271

EP - 278

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -