The lactoperoxidase system functions in bacterial clearance of airways

Cynthia Gerson, Juan Sabater, Mario Scuri, Aliza Torbati, Richard Coffey, Jeffrey W. Abraham, Isabel Lauredo, Rosanna Forteza, Adam Wanner, Matthias Salathe, William M. Abraham, Gregory E. Conner

Research output: Contribution to journalArticlepeer-review

143 Scopus citations


Airway mucus is a complex mixture of secretory products that provides a multifaceted defense against pulmonary infection. Mucus contains antimicrobial peptides (e.g., defensins) and enzymes (e.g., lysozyme) although the contribution of these to airway sterility has not been tested in vivo. We have previously shown that an enzymatically active, heme-containing peroxidase comprises 1% of the soluble protein in sheep airway secretions, and it has been hypothesized that this airway peroxidase may function as a biocidal system. In this study, we show that sheep airway peroxidase is identical to milk lactoperoxidase (LPO) and that sheep airway secretions contain thiocyanate (SCN-) at concentrations necessary and sufficient for a functional peroxidase system that can protect against infection. We also show that airway LPO, like milk LPO, produces the biocidal compound hypothiocyanite (OSCN-) in vitro. Finally, we show that in vivo inhibition of airway LPO in sheep leads to a significant decrease in bacterial clearance from the airways. The data suggest that the LPO system is a major contributor to airway defenses. This discovery may have significant implications for chronic airway colonization seen in respiratory diseases such as cystic fibrosis.

Original languageEnglish (US)
Pages (from-to)665-671
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Issue number6
StatePublished - 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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