The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila

Lina Ni, Mason Klein, Kathryn V. Svec, Gonzalo Budelli, Elaine C. Chang, Anggie J. Ferrer, Richard Benton, Aravinthan D T Samuel, Paul A. Garrity

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Animals rely on highly sensitive thermoreceptors to seek out optimal temperatures, but the molecular mechanisms of thermosensing are not well understood. The Dorsal Organ Cool Cells (DOCCs) of the Drosophila larva are a set of exceptionally thermosensitive neurons critical for larval cool avoidance. Here, we show that DOCC cool-sensing is mediated by Ionotropic Receptors (IRs), a family of sensory receptors widely studied in invertebrate chemical sensing. We find that two IRs, IR21a and IR25a, are required to mediate DOCC responses to cooling and are required for cool avoidance behavior. Furthermore, we find that ectopic expression of IR21a can confer coolresponsiveness in an Ir25a-dependent manner, suggesting an instructive role for IR21a in thermosensing. Together, these data show that IR family receptors can function together to mediate thermosensation of exquisite sensitivity.

Original languageEnglish (US)
Article numbere13254
JournaleLife
Volume5
Issue numberAPRIL2016
DOIs
StatePublished - Apr 29 2016

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Thermosensing
Drosophila
Neurons
Animals
Cooling
Thermoreceptors
Avoidance Learning
Sensory Receptor Cells
Invertebrates
Temperature
Larva
Cool-X-A

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Ni, L., Klein, M., Svec, K. V., Budelli, G., Chang, E. C., Ferrer, A. J., ... Garrity, P. A. (2016). The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila. eLife, 5(APRIL2016), [e13254]. https://doi.org/10.7554/eLife.13254

The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila. / Ni, Lina; Klein, Mason; Svec, Kathryn V.; Budelli, Gonzalo; Chang, Elaine C.; Ferrer, Anggie J.; Benton, Richard; Samuel, Aravinthan D T; Garrity, Paul A.

In: eLife, Vol. 5, No. APRIL2016, e13254, 29.04.2016.

Research output: Contribution to journalArticle

Ni, L, Klein, M, Svec, KV, Budelli, G, Chang, EC, Ferrer, AJ, Benton, R, Samuel, ADT & Garrity, PA 2016, 'The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila', eLife, vol. 5, no. APRIL2016, e13254. https://doi.org/10.7554/eLife.13254
Ni L, Klein M, Svec KV, Budelli G, Chang EC, Ferrer AJ et al. The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila. eLife. 2016 Apr 29;5(APRIL2016). e13254. https://doi.org/10.7554/eLife.13254
Ni, Lina ; Klein, Mason ; Svec, Kathryn V. ; Budelli, Gonzalo ; Chang, Elaine C. ; Ferrer, Anggie J. ; Benton, Richard ; Samuel, Aravinthan D T ; Garrity, Paul A. / The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila. In: eLife. 2016 ; Vol. 5, No. APRIL2016.
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