The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure

Isabelle Callebaut, Cyntia Curcio-Morelli, Jean P. Mornon, Balazs Gereben, Christoph Buettner, Stephen Huang, Bertrand Castro, Tatiana L. Fonseca, John W. Harney, P. Reed Larsen, Antonio C. Bianco

Research output: Contribution to journalArticle

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Abstract

The three iodothyronine selenodeiodinases catalyze the initiation and termination of thyroid hormone effects in vertebrates. Structural analyses of these proteins have been hindered by their integral membrane nature and the inefficient eukaryotic-specific pathway for selenoprotein synthesis. Hydrophobic cluster analysis used in combination with Position-specific Iterated BLAST reveals that their extramembrane portion belongs to the thioredoxin-fold superfamily for which experimental structure information exists. Moreover, a large deiodinase region imbedded in the thioredoxin fold shares strong similarities with the active site of iduronidase, a member of the clan GH-A-fold of glycoside hydrolases. This model can explain a number of results from previous mutagenesis analyses and permits new verifiable insights into the structural and functional properties of these enzymes.

Original languageEnglish
Pages (from-to)36887-36896
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number38
DOIs
StatePublished - Sep 19 2003

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Thioredoxins
Glycoside Hydrolases
Iduronidase
Selenoproteins
Iodide Peroxidase
Mutagenesis
Cluster analysis
Thyroid Hormones
Cluster Analysis
Vertebrates
Catalytic Domain
Proteins
Membranes
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Callebaut, I., Curcio-Morelli, C., Mornon, J. P., Gereben, B., Buettner, C., Huang, S., ... Bianco, A. C. (2003). The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure. Journal of Biological Chemistry, 278(38), 36887-36896. https://doi.org/10.1074/jbc.M305725200

The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure. / Callebaut, Isabelle; Curcio-Morelli, Cyntia; Mornon, Jean P.; Gereben, Balazs; Buettner, Christoph; Huang, Stephen; Castro, Bertrand; Fonseca, Tatiana L.; Harney, John W.; Larsen, P. Reed; Bianco, Antonio C.

In: Journal of Biological Chemistry, Vol. 278, No. 38, 19.09.2003, p. 36887-36896.

Research output: Contribution to journalArticle

Callebaut, I, Curcio-Morelli, C, Mornon, JP, Gereben, B, Buettner, C, Huang, S, Castro, B, Fonseca, TL, Harney, JW, Larsen, PR & Bianco, AC 2003, 'The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure', Journal of Biological Chemistry, vol. 278, no. 38, pp. 36887-36896. https://doi.org/10.1074/jbc.M305725200
Callebaut, Isabelle ; Curcio-Morelli, Cyntia ; Mornon, Jean P. ; Gereben, Balazs ; Buettner, Christoph ; Huang, Stephen ; Castro, Bertrand ; Fonseca, Tatiana L. ; Harney, John W. ; Larsen, P. Reed ; Bianco, Antonio C. / The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 38. pp. 36887-36896.
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