The involvement of H+-ATPase and carbonic anhydrase in intestinal HCO3- secretion in seawater-acclimated rainbow trout

Martin Grosell, J. Genz, J. R. Taylor, S. F. Perry, K. M. Gilmour

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Pyloric caeca and anterior intestine epithelia from seawater-accli mated rainbow trout exhibit different electrophysiological parameters with lower transepithelial potential and higher epithelial conductance in the pyloric caeca than the anterior intestine. Both pyloric caeca and the anterior intestine secrete HCO3- at high rates in the absence of serosal HCO3-/CO2, demonstrating that endogenous CO2 is the principal source of HCO3- under resting control conditions. Apical, bafilomycin-sensitive, H+ extrusion occurs in the anterior intestine and probably acts to control luminal osmotic pressure while enhancing apical anion exchange; both processes with implications for water absorption. Cytosolic carbonic anhydrase (CAc) activity facilitates CO2 hydration to fuel apical anion exchange while membrane-associated, luminal CA activity probably facilitates the conversion of HCO3- to CO2. The significance of membrane-bound, luminal CA may be in part to reduce HCO3- gradients across the apical membrane to further enhance anion exchange and thus Cl- absorption and to facilitate the substantial CaCO3 precipitation occurring in the lumen of marine teleosts. In this way, membrane-bound, luminal CA thus promotes the absorption of osmolytes and reduction on luminal osmotic pressure, both of which will serve to enhance osmotic gradients to promote intestinal water absorption.

Original languageEnglish
Pages (from-to)1940-1948
Number of pages9
JournalJournal of Experimental Biology
Volume212
Issue number12
DOIs
StatePublished - Jun 15 2009

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Intestinal Secretions
H-transporting ATP synthase
Proton-Translocating ATPases
Carbonic Anhydrases
Oncorhynchus mykiss
carbonate dehydratase
Seawater
secretion
rainbow
Intestines
intestines
anion exchange
seawater
cecum
membrane
Anions
ion exchange
Membranes
Osmotic Pressure
osmotic pressure

Keywords

  • Bicarbonate transport
  • Marine fish
  • Osmoregulation
  • Proton pump
  • Water absorption

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

The involvement of H+-ATPase and carbonic anhydrase in intestinal HCO3- secretion in seawater-acclimated rainbow trout. / Grosell, Martin; Genz, J.; Taylor, J. R.; Perry, S. F.; Gilmour, K. M.

In: Journal of Experimental Biology, Vol. 212, No. 12, 15.06.2009, p. 1940-1948.

Research output: Contribution to journalArticle

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