The interaction of bisulfite and its free radical analog-nitroxyldisulfonate with periodic acid-oxidized lymphocytes and their effect on blastogenesis

Francis J. Hornicek, George I. Malinin, Howard G. Gratzner, Theodore I. Malinin

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Nitroxyldisulfonate [Fremy's salt; (KSO3)2NO] and bisulfite (NaHSO3) have abolished periodic acid (H5IO6)-induced blastogenesis of human peripheral blood lymphocytes (HPBL), but only inhibited the blastogenic response of H5IO6-oxidized rat and mouse lymphocytes, as determined by the rates of nucleic acids synthesis, BrdUrd incorporation and by cell numbers in S + G2 + M phases of the cell cycle. The viability of the intact human, rat and mouse lymphocytes remained essentially unimpaired by 30 min pulses of 1 mM Fremy's salt or bisulfite. The marked inhibition of periodic acidinduced blastogenesis, exerted by Fremy's salt and by bisulfite, was attributed to the effect of the corresponding carbonyl addition derivatives formed in situ of the oxidized cell membranes. Consequently, it is concluded that Fremy's salt like bisulfite possibly forms addition derivatives with membrane carbonyls of viable target cells.

Original languageEnglish (US)
Pages (from-to)289-302
Number of pages14
JournalChemico-Biological Interactions
Volume55
Issue numberC
DOIs
StatePublished - 1985

Keywords

  • Bisulfite
  • Blastogenesis
  • Carbonyls
  • Free radicals
  • Fremy's salt
  • Lymphocytes
  • Periodic acid
  • Spin-labeling
  • Viability

ASJC Scopus subject areas

  • Toxicology

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