The insulin-like growth factor binding protein BP-25 is expressed by human breast cancer cells

D. Yee, R. E. Favoni, R. Lupu, K. J. Cullen, G. S. Lebovic, K. K. Huff, P. D K Lee, Y. L. Lee, D. R. Powell, R. B. Dickson, N. Rosen, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

Specific binding proteins are thought to modulate the effects of IGF-I. Previous work has demonstrated that media conditioned by human breast cancer cells contains IGF-I binding activity. Radiolabelled IGF-I incubated with serum-free conditioned media from the breast cancer cell line MDA-MB 231 eluted with an apparent M.W. of 35-40 kDa when analyzed by gel filtration chromatography at pH 7.4. The M.W. of this binding activity corresponded to that of BP-25, a binding protein cloned from the hepatocellular carcinoma cell line HepG2. Two breast cancer cell lines, MDA-MB 231 and Hs578T, were found to express BP-25 RNA. Specific BP-25 radioimmunoassay detected BP-25 production in the conditioned media of these two cell lines. Immunoprecipitation confirmed that metabolically labelled MDA-MB 231 released 30 kDa BP-25 into its medium. This study demonstrates that some breast cancer cells express the IGF-I binding protein, BP-25.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number1
DOIs
StatePublished - Jan 16 1989
Externally publishedYes

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Insulin-Like Growth Factor Binding Proteins
Insulin-Like Growth Factor I
Conditioned Culture Medium
Cells
Breast Neoplasms
Cell Line
Carrier Proteins
Serum-Free Culture Media
Immunoprecipitation
Radioimmunoassay
Gel Chromatography
Hepatocellular Carcinoma
RNA
Chromatography
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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The insulin-like growth factor binding protein BP-25 is expressed by human breast cancer cells. / Yee, D.; Favoni, R. E.; Lupu, R.; Cullen, K. J.; Lebovic, G. S.; Huff, K. K.; Lee, P. D K; Lee, Y. L.; Powell, D. R.; Dickson, R. B.; Rosen, N.; Lippman, Marc E.

In: Biochemical and Biophysical Research Communications, Vol. 158, No. 1, 16.01.1989, p. 38-44.

Research output: Contribution to journalArticle

Yee, D, Favoni, RE, Lupu, R, Cullen, KJ, Lebovic, GS, Huff, KK, Lee, PDK, Lee, YL, Powell, DR, Dickson, RB, Rosen, N & Lippman, ME 1989, 'The insulin-like growth factor binding protein BP-25 is expressed by human breast cancer cells', Biochemical and Biophysical Research Communications, vol. 158, no. 1, pp. 38-44. https://doi.org/10.1016/S0006-291X(89)80173-1
Yee, D. ; Favoni, R. E. ; Lupu, R. ; Cullen, K. J. ; Lebovic, G. S. ; Huff, K. K. ; Lee, P. D K ; Lee, Y. L. ; Powell, D. R. ; Dickson, R. B. ; Rosen, N. ; Lippman, Marc E. / The insulin-like growth factor binding protein BP-25 is expressed by human breast cancer cells. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 158, No. 1. pp. 38-44.
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