The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity

Jean Marc Jacque, Mario Stevenson

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Primate lentiviruses such as human immunodeficiency type 1 (HIV-1) have the capacity to infect non-dividing cells such as tissue macrophages. In the process, viral complementary DNA traverses the nuclear envelope to integrate within chromatin. Given the intimate association between chromatin and the nuclear envelope, we examined whether HIV-1 appropriates nuclear envelope components during infection. Here we show that emerin, an integral inner-nuclear-envelope protein, is necessary for HIV-1 infection. Infection of primary macrophages lacking emerin was abortive in that viral cDNA localized to the nucleus but integration into chromatin was inefficient, and conversion of viral cDNA to non-functional episomal cDNA increased. HIV-1 cDNA associated with emerin in vivo, and the interaction of viral cDNA with chromatin was dependent on emerin. Barrier-to-autointegration factor (BAF), the LEM (LAP, emerin, MAN) binding partner of emerin, was required for the association of viral cDNA with emerin and for the ability of emerin to support virus infection. Therefore emerin, which bridges the interface between the inner nuclear envelope and chromatin, may be necessary for chromatin engagement by viral cDNA before integration.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalNature
Volume441
Issue number7093
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

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Nuclear Envelope
Nuclear Proteins
HIV-1
Complementary DNA
Chromatin
Primate Lentiviruses
Macrophages
emerin
Viral DNA
Virus Diseases
Infection
HIV Infections

ASJC Scopus subject areas

  • General

Cite this

The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity. / Jacque, Jean Marc; Stevenson, Mario.

In: Nature, Vol. 441, No. 7093, 01.06.2006, p. 641-645.

Research output: Contribution to journalArticle

Jacque, Jean Marc ; Stevenson, Mario. / The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity. In: Nature. 2006 ; Vol. 441, No. 7093. pp. 641-645.
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