The inheritance of glomerulosclerosis in mice is controlled by multiple quantitative trait loci

Oliver Lenz, Feng Zheng, Jose Vilar, Sophie Doublier, Enrico Lupia, Susanne Schwedler, Liliane J. Striker, Gary E. Striker

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background. Glomerulosclerosis, the common terminal event in chronic glomerular diseases such as diabetic nephropathy or IgA nephropathy, leads to end-stage renal disease. The considerable variation in both the risk of developing glomerulosclerosis and the rate of progression in individual patients suggest a role for genetic factors which have not been identified so far. In this study we sought to examine the mode of inheritance of glomerulosclerosis in mice. Methods. F1 animals of a mating between glomerulosclerosis-prone ROP-Os/+ male and non-sclerotic C3H female mice were backcrossed to the ROP strain. We took advantage of the radiation-induced mutation oligosyndactylism (Os) to identify glomerulosclerosis at the age of 3 months. Kidneys were perfused in situ with PBS/Formalin 10%. The extent of glomerulosclerotic lesions was evaluated on PAS stained paraffin sections using computer-aided morphometry. Results. F1 mice did not show any glomerulosclerosis. In the backcross offspring, we found a wide distribution of glomerular lesions between individual animals, ranging from normal to very severe. We calculated that at least 8-10 loci determine the severity of glomerulosclerosis in mice. Conclusions. Our data show that glomerulosclerosis is inherited in a recessive fashion involving at least 8-10 loci.

Original languageEnglish
Pages (from-to)3074-3078
Number of pages5
JournalNephrology Dialysis Transplantation
Volume13
Issue number12
StatePublished - Dec 1 1998
Externally publishedYes

    Fingerprint

Keywords

  • Genetics
  • Glomerulosclerosis
  • Mice
  • Oligosyndactyly

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Lenz, O., Zheng, F., Vilar, J., Doublier, S., Lupia, E., Schwedler, S., Striker, L. J., & Striker, G. E. (1998). The inheritance of glomerulosclerosis in mice is controlled by multiple quantitative trait loci. Nephrology Dialysis Transplantation, 13(12), 3074-3078.