The influence of glucose on intracellular and extracellular pH in rat hippocampal slices during and after anoxia

Eugene L. Roberts, Jiao He, Ching Ping Chih

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


In this study we investigated in rat hippocampal slices (1) how glucose availability affected tissue acidosis during and after anoxia, (2) whether the onset of anoxic depolarization was associated with a specific pH, (3) whether glycolysis was the major source of acidification before and during anoxic depolarization, and (4) whether improved recovery of synaptic function with elevated glucose levels was related to changes in tissue acidosis. Intracellular pH (pH(i)) and extracellular pH (pH(o)) were measured simultaneously before, during, and after anoxia in hippocampal slices bathed in 0, 5, 10, and 15 mM glucose. Slices exposed to 0 mM glucose were given 20 mM sodium lactate as a metabolic substrate. We found that the pH(i) and pH(o) at which anoxic depolarization occurred depended upon glucose concentration. We also found that elevated glucose availability increased acidification in both the intracellular and extracellular compartments during anoxia and delayed recovery of pH homeostasis after anoxia. Our results suggest that glycolysis is the primary source of acidosis before the onset of anoxic depolarization, but not during anoxic depolarization. Our results also suggest that moderate increased in acidosis resulting from increased glycolysis are potentially beneficial for anoxic survival.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalBrain research
Issue number1
StatePublished - Feb 2 1998


  • Anoxic depolarization
  • Brain slice
  • Energy metabolism
  • Hydrogen ion concentration
  • Hypoxia
  • Lactate
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)


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