The influence of electrospun aligned poly(ε-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes

Jin San Choi, Sang Jin Lee, George J. Christ, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

402 Citations (Scopus)

Abstract

Current treatment options for restoring large skeletal muscle tissue defects due to trauma or tumor ablation are limited by the host muscle tissue availability and donor site morbidity of muscle flap implantation. Creation of implantable functional muscle tissue that could restore muscle defects may bea possible solution. To engineer functional muscle tissue for reconstruction, scaffolds that mimic native fibers need to be developed. In this study we examined the feasibility of using poly(ε-caprolactone) (PCL)/collagen based nanofibers using electrospinning as a scaffold system for implantable engineered muscle. We investigated whether electrospun nanofibers could guide morphogenesis of skeletal muscle cells and enhance cellular organization. Nanofibers with different fiber orientations were fabricated by electrospinning with a blend of PCL and collagen. Human skeletal muscle cells (hSkMCs) were seeded onto the electrospun PCL/collagen nanofiber meshes and analyzed for cell adhesion, proliferation and organization. Our results show that unidirectionally oriented nanofibers significantly induced muscle cell alignment and myotube formation as compared to randomly oriented nanofibers. The aligned composite nanofiber scaffolds seeded with skeletal muscle cells may provide implantable functional muscle tissues for patients with large muscle defects.

Original languageEnglish
Pages (from-to)2899-2906
Number of pages8
JournalBiomaterials
Volume29
Issue number19
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

Fingerprint

Nanofibers
Skeletal Muscle Fibers
Collagen
Muscle
Skeletal Muscle
Muscles
Muscle Cells
Tissue
Cells
Electrospinning
Tissue Scaffolds
Scaffolds
polycaprolactone
Defects
Feasibility Studies
Morphogenesis
Textile blends
Cell Adhesion
Cell adhesion
Cell Proliferation

Keywords

  • Cell alignment
  • Collagen
  • Electrospinning
  • Myotube formation
  • Polycaprolactone
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The influence of electrospun aligned poly(ε-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. / Choi, Jin San; Lee, Sang Jin; Christ, George J.; Atala, Anthony; Yoo, James J.

In: Biomaterials, Vol. 29, No. 19, 01.07.2008, p. 2899-2906.

Research output: Contribution to journalArticle

Choi, Jin San ; Lee, Sang Jin ; Christ, George J. ; Atala, Anthony ; Yoo, James J. / The influence of electrospun aligned poly(ε-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. In: Biomaterials. 2008 ; Vol. 29, No. 19. pp. 2899-2906.
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