The Influence of Age on Serum Concentrations of Cardiac Troponin I:Results in Rats, Monkeys, and Commercial Sera

Eugene H. Herman, Alan Knapton, Yongmin Liu, Steven E. Lipshultz, Joel Estis, John Todd, Ruth A. Woodward, Thomas Cochran, Jun Zhang, Miriam C. Poirier

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Cardiac troponins serve as serum biomarkers of myocardial injury. The current study examined the influence of age on serum concentrations of cardiac troponin I (cTnI). An ultrasensitive immunoassay was used to monitor cTnI concentrations in Sprague-Dawley (SD) rats and Erythrocebus patas monkeys of different ages. The mean cTnI concentrations were highest in 10-day-old rats compared to 25-, 40-, and 80-day-old SD rats. Cardiomyocyte remodeling was apparent in hearts from 10-day-old SD rats as evident by hypercellularity, irregularly shaped nuclei, and moderate numbers of myocytes undergoing mitosis and apoptosis. The mean concentration of cTnI in 5 newborn monkeys was considerably higher than that of three 1-year-old monkeys. Evidence of cardiomyocyte remodeling was also observed in these newborn hearts (loss of myofibrils and cytoplasmic vacuolation). Commercial animal serum samples were also analyzed. The concentrations of cTnI detected in fetal equine and porcine serum were considerably higher than that found in adult equine and porcine serum samples Likewise, fetal bovine serum had higher cTnI concentrations (>2,400 pg/ml) than did adult caprine and laprine samples (2.5-2.7 pg/ml). The present study found age-related differences in cTnI concentrations, with higher levels occurring at younger ages. This effect was consistent across several animal species.

Original languageEnglish (US)
Pages (from-to)888-896
Number of pages9
JournalToxicologic Pathology
Volume42
Issue number5
DOIs
StatePublished - Jul 2014

Keywords

  • cardiac troponin I
  • patas monkeys
  • Sprague-Dawley rats
  • young age.

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology
  • Molecular Biology

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    Herman, E. H., Knapton, A., Liu, Y., Lipshultz, S. E., Estis, J., Todd, J., Woodward, R. A., Cochran, T., Zhang, J., & Poirier, M. C. (2014). The Influence of Age on Serum Concentrations of Cardiac Troponin I:Results in Rats, Monkeys, and Commercial Sera. Toxicologic Pathology, 42(5), 888-896. https://doi.org/10.1177/0192623313505154