Abstract
Purpose: To determine the impact of flap creation methods for sub-Bowman's keratomileusis (SBK) on central Bowman 's layer thickness. Methods: SBK flaps were made by Moria microkeratome for 20 subjects and by femtosecond (FEMTO) laser for 21 subjects. Corneal sublayer thicknesses were measured by ultra-high resolution optical coherence tomography before SBK and at 1 day, 1 week, 2 weeks, and 1 month afterwards. Each subject was imaged twice on each visit. Thicknesses of central epithelium, Bowman's layer, flap, and total cornea were calculated using a custom-made automated image processing algorithm. The repeatability of sublayer thickness measurements was tested by the intraclass correlation coefficient (ICC) and by the coefficient of repeatability (CoR) at 1 week post-SBK. Results: ICCs of the Moria and FEMTO groups were ≥0.959 and ≥0.961 respectively for all sublayer measurements. The segmentation CoRs were less than 6.78% and 5.63%respectively. For both groups, microdistortions were present in the epithelium and Bowman's layer after SKB. The flap thickness of the Moria group was 9.8 μ m (95% confidence interval: 4.8-14.8μm) thinner than the FEMTO group one day after SBK (independent samples t-test, P <0.05). Bowman's layer became thicker by 1.6 ± 1.1 μm and 1.7 ± 1.6 μm one day post-SBK for the Moria and FEMTO groups (repeated ANOVA, P <0.05) and then remained stable. Corneal and sublayer thickness were similar between the two groups. Conclusions: Central Bowman's layer thickness increased 1 day post-SBK. Flap creation by Moria microkeratome and femtosecond laser did not have significantly different impacts on Bowman's layer thickness following SBK. Trial Registration: Chinese Clinical Trial Registry (ChiCTR) NO: ChiCTR-OCH-14004525.
| Original language | English (US) |
|---|---|
| Article number | e0124996 |
| Journal | PLoS One |
| Volume | 10 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 4 2015 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
The impact of flap creation methods for Sub-Bowman's Keratomileusis (SBK) on the central thickness of Bowman's layer. / Xu, Zhe; Shen, Meixiao; Hu, Liang; Zhuang, Xiran; Peng, Mei; Hu, Di; Liu, Jing; Wang, Jianhua; Qu, Jia; Lu, Fan.
In: PLoS One, Vol. 10, No. 5, e0124996, 04.05.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The impact of flap creation methods for Sub-Bowman's Keratomileusis (SBK) on the central thickness of Bowman's layer
AU - Xu, Zhe
AU - Shen, Meixiao
AU - Hu, Liang
AU - Zhuang, Xiran
AU - Peng, Mei
AU - Hu, Di
AU - Liu, Jing
AU - Wang, Jianhua
AU - Qu, Jia
AU - Lu, Fan
PY - 2015/5/4
Y1 - 2015/5/4
N2 - Purpose: To determine the impact of flap creation methods for sub-Bowman's keratomileusis (SBK) on central Bowman 's layer thickness. Methods: SBK flaps were made by Moria microkeratome for 20 subjects and by femtosecond (FEMTO) laser for 21 subjects. Corneal sublayer thicknesses were measured by ultra-high resolution optical coherence tomography before SBK and at 1 day, 1 week, 2 weeks, and 1 month afterwards. Each subject was imaged twice on each visit. Thicknesses of central epithelium, Bowman's layer, flap, and total cornea were calculated using a custom-made automated image processing algorithm. The repeatability of sublayer thickness measurements was tested by the intraclass correlation coefficient (ICC) and by the coefficient of repeatability (CoR) at 1 week post-SBK. Results: ICCs of the Moria and FEMTO groups were ≥0.959 and ≥0.961 respectively for all sublayer measurements. The segmentation CoRs were less than 6.78% and 5.63%respectively. For both groups, microdistortions were present in the epithelium and Bowman's layer after SKB. The flap thickness of the Moria group was 9.8 μ m (95% confidence interval: 4.8-14.8μm) thinner than the FEMTO group one day after SBK (independent samples t-test, P <0.05). Bowman's layer became thicker by 1.6 ± 1.1 μm and 1.7 ± 1.6 μm one day post-SBK for the Moria and FEMTO groups (repeated ANOVA, P <0.05) and then remained stable. Corneal and sublayer thickness were similar between the two groups. Conclusions: Central Bowman's layer thickness increased 1 day post-SBK. Flap creation by Moria microkeratome and femtosecond laser did not have significantly different impacts on Bowman's layer thickness following SBK. Trial Registration: Chinese Clinical Trial Registry (ChiCTR) NO: ChiCTR-OCH-14004525.
AB - Purpose: To determine the impact of flap creation methods for sub-Bowman's keratomileusis (SBK) on central Bowman 's layer thickness. Methods: SBK flaps were made by Moria microkeratome for 20 subjects and by femtosecond (FEMTO) laser for 21 subjects. Corneal sublayer thicknesses were measured by ultra-high resolution optical coherence tomography before SBK and at 1 day, 1 week, 2 weeks, and 1 month afterwards. Each subject was imaged twice on each visit. Thicknesses of central epithelium, Bowman's layer, flap, and total cornea were calculated using a custom-made automated image processing algorithm. The repeatability of sublayer thickness measurements was tested by the intraclass correlation coefficient (ICC) and by the coefficient of repeatability (CoR) at 1 week post-SBK. Results: ICCs of the Moria and FEMTO groups were ≥0.959 and ≥0.961 respectively for all sublayer measurements. The segmentation CoRs were less than 6.78% and 5.63%respectively. For both groups, microdistortions were present in the epithelium and Bowman's layer after SKB. The flap thickness of the Moria group was 9.8 μ m (95% confidence interval: 4.8-14.8μm) thinner than the FEMTO group one day after SBK (independent samples t-test, P <0.05). Bowman's layer became thicker by 1.6 ± 1.1 μm and 1.7 ± 1.6 μm one day post-SBK for the Moria and FEMTO groups (repeated ANOVA, P <0.05) and then remained stable. Corneal and sublayer thickness were similar between the two groups. Conclusions: Central Bowman's layer thickness increased 1 day post-SBK. Flap creation by Moria microkeratome and femtosecond laser did not have significantly different impacts on Bowman's layer thickness following SBK. Trial Registration: Chinese Clinical Trial Registry (ChiCTR) NO: ChiCTR-OCH-14004525.
UR - http://www.scopus.com/inward/record.url?scp=84929119498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929119498&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0124996
DO - 10.1371/journal.pone.0124996
M3 - Article
C2 - 25938492
AN - SCOPUS:84929119498
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 5
M1 - e0124996
ER -