The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus

Zdena Kohoutová, Michaela Rumlová, Martin Andreansky, Michael Sakalian, Eric Hunter, Iva Pichová, Tomáš Ruml

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the "Rous-like" virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the "HIV-like" virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the "HIV-like", which retained fractional infectivity.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalVirology
Volume384
Issue number1
DOIs
StatePublished - Feb 5 2009
Externally publishedYes

Fingerprint

Mason-Pfizer monkey virus
Polyproteins
HIV
Retroviridae
pol Gene Products
gag Gene Products
Virus Assembly
Rous sarcoma virus
Morphogenesis
Viruses
Enzymes
Proteins

Keywords

  • Assembly
  • Capsid
  • Mason-Pfizer monkey virus
  • Retrovirus
  • Ribosomal frameshift

ASJC Scopus subject areas

  • Virology

Cite this

The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. / Kohoutová, Zdena; Rumlová, Michaela; Andreansky, Martin; Sakalian, Michael; Hunter, Eric; Pichová, Iva; Ruml, Tomáš.

In: Virology, Vol. 384, No. 1, 05.02.2009, p. 59-68.

Research output: Contribution to journalArticle

Kohoutová, Z, Rumlová, M, Andreansky, M, Sakalian, M, Hunter, E, Pichová, I & Ruml, T 2009, 'The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus', Virology, vol. 384, no. 1, pp. 59-68. https://doi.org/10.1016/j.virol.2008.10.048
Kohoutová, Zdena ; Rumlová, Michaela ; Andreansky, Martin ; Sakalian, Michael ; Hunter, Eric ; Pichová, Iva ; Ruml, Tomáš. / The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. In: Virology. 2009 ; Vol. 384, No. 1. pp. 59-68.
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