The imidazoline derivative calmidazolium inhibits voltage-gated Ca2+-channels and insulin release but has no effect on the phospholipase C system in insulin producing RINm5F-cells

Henrik Kindmark, Martin Köhler, Pär Gerwins, Olof Larsson, Akhtar Khan, Martin A. Wahl, Per Olof Berggren

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present study shows that the calmodulin antagonist calmidazolium inhibited influx of Ca2+ through voltage-gated Ca2+-channels in clonal insulin producing RINm5F-cells. The mechanism of inhibition may involve both Ca2+-calmodulin-dependent protein kinases and direct binding of calmidazolium to the Ca2+-channel. Calmidazolium did not affect uptake of Ca2+ into intracellular Ca2+-pools, inositol 1,4,5-trisphosphate (InsP3) formation or action on intracellular Ca2+-pools. The calmodulin inhibitor also did not affect glucose utilization or oxidation in RINm5F-cells, speaking against an unspecific toxic effect of the compound. KCl-and ATP-stimulated insulin release from RINm5F-cells was attenuated by calmidazolium, whereas basal hormone secretion was unaffected.

Original languageEnglish
Pages (from-to)145-158
Number of pages14
JournalBioscience Reports
Volume14
Issue number3
DOIs
StatePublished - Jun 1 1994
Externally publishedYes

Fingerprint

calmidazolium
Imidazolines
Type C Phospholipases
Insulin
Derivatives
Electric potential
Calmodulin
Calcium-Calmodulin-Dependent Protein Kinases
Inositol 1,4,5-Trisphosphate
Poisons
Corrosion inhibitors
Adenosine Triphosphate
Hormones
Glucose
Oxidation

Keywords

  • Ca-channels
  • calmidazolium
  • calmodulin inhibitor
  • insulin release
  • RINm5F-cells

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The imidazoline derivative calmidazolium inhibits voltage-gated Ca2+-channels and insulin release but has no effect on the phospholipase C system in insulin producing RINm5F-cells. / Kindmark, Henrik; Köhler, Martin; Gerwins, Pär; Larsson, Olof; Khan, Akhtar; Wahl, Martin A.; Berggren, Per Olof.

In: Bioscience Reports, Vol. 14, No. 3, 01.06.1994, p. 145-158.

Research output: Contribution to journalArticle

Kindmark, Henrik ; Köhler, Martin ; Gerwins, Pär ; Larsson, Olof ; Khan, Akhtar ; Wahl, Martin A. ; Berggren, Per Olof. / The imidazoline derivative calmidazolium inhibits voltage-gated Ca2+-channels and insulin release but has no effect on the phospholipase C system in insulin producing RINm5F-cells. In: Bioscience Reports. 1994 ; Vol. 14, No. 3. pp. 145-158.
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