The identification and characterization of KRAB-domain-containing zinc finger proteins

C. D. Consiantinou-Deltas, John Gilbert, R. J. Bartlett, M. Herbstreith, A. D. Roses, J. E. Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The zinc finger motif is a highly conserved tandemly repeated sequence of 28-30 amino acids that was first identified in transcription factor TFIIIA from Xenopus laevis. Subsequently, similar motifs were found and characterized in many genes from mammalian genomes and the genomes of lower eukaryotes such as Drosophila and yeast, thereby defining a large superfamily of genes. Non-finger-coding modules conserved among members of subfamilies of zinc finger genes have been described in the murine genome (finger-associated boxes, or FAX domain) and the human genome (Krüppel-associated boxes, or KRAB domain). Here we report the identification and partial characterization of more members of the human KRAB-containing subfamily of genes. Based on Southern blot hybridization experiments, they also are zinc-finger-coding genes. All members share a highly homologous 42-amino-acid-long a element of the described KRAB domain. The conservation extends to the murine developmentally expressed zinc finger gene, mKr2. The homologous sequences, however, are part of the 5′-untranslated region. In all cases for which there is adequate information, the KRAB domain is found at the NH2-terminus of the respective protein. In one zinc-finger-encoding cDNA clone that we characterized further in this work, BRc1744 (ZNF45), the KRAB domain most probably constitutes the entire second exon of the gene. Based on the data, it is tempting to speculate that the FAX- and KRAB-containing zinc finger genes define subfamilies of genes with overlapping functions that participate in the regulation of common or similar developmental programs.

Original languageEnglish
Pages (from-to)581-589
Number of pages9
JournalGenomics
Volume12
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Zinc Fingers
Genes
Proteins
Genome
Transcription Factor TFIIIA
Overlapping Genes
Amino Acids
5' Untranslated Regions
Xenopus laevis
Human Genome
Sequence Homology
Southern Blotting
Eukaryota
Fingers
Drosophila
Exons
Complementary DNA
Clone Cells
Yeasts

ASJC Scopus subject areas

  • Genetics

Cite this

Consiantinou-Deltas, C. D., Gilbert, J., Bartlett, R. J., Herbstreith, M., Roses, A. D., & Lee, J. E. (1992). The identification and characterization of KRAB-domain-containing zinc finger proteins. Genomics, 12(3), 581-589. https://doi.org/10.1016/0888-7543(92)90451-W

The identification and characterization of KRAB-domain-containing zinc finger proteins. / Consiantinou-Deltas, C. D.; Gilbert, John; Bartlett, R. J.; Herbstreith, M.; Roses, A. D.; Lee, J. E.

In: Genomics, Vol. 12, No. 3, 01.01.1992, p. 581-589.

Research output: Contribution to journalArticle

Consiantinou-Deltas, CD, Gilbert, J, Bartlett, RJ, Herbstreith, M, Roses, AD & Lee, JE 1992, 'The identification and characterization of KRAB-domain-containing zinc finger proteins', Genomics, vol. 12, no. 3, pp. 581-589. https://doi.org/10.1016/0888-7543(92)90451-W
Consiantinou-Deltas CD, Gilbert J, Bartlett RJ, Herbstreith M, Roses AD, Lee JE. The identification and characterization of KRAB-domain-containing zinc finger proteins. Genomics. 1992 Jan 1;12(3):581-589. https://doi.org/10.1016/0888-7543(92)90451-W
Consiantinou-Deltas, C. D. ; Gilbert, John ; Bartlett, R. J. ; Herbstreith, M. ; Roses, A. D. ; Lee, J. E. / The identification and characterization of KRAB-domain-containing zinc finger proteins. In: Genomics. 1992 ; Vol. 12, No. 3. pp. 581-589.
@article{414430a48c8f4a41ba7f3a46781d5844,
title = "The identification and characterization of KRAB-domain-containing zinc finger proteins",
abstract = "The zinc finger motif is a highly conserved tandemly repeated sequence of 28-30 amino acids that was first identified in transcription factor TFIIIA from Xenopus laevis. Subsequently, similar motifs were found and characterized in many genes from mammalian genomes and the genomes of lower eukaryotes such as Drosophila and yeast, thereby defining a large superfamily of genes. Non-finger-coding modules conserved among members of subfamilies of zinc finger genes have been described in the murine genome (finger-associated boxes, or FAX domain) and the human genome (Kr{\"u}ppel-associated boxes, or KRAB domain). Here we report the identification and partial characterization of more members of the human KRAB-containing subfamily of genes. Based on Southern blot hybridization experiments, they also are zinc-finger-coding genes. All members share a highly homologous 42-amino-acid-long a element of the described KRAB domain. The conservation extends to the murine developmentally expressed zinc finger gene, mKr2. The homologous sequences, however, are part of the 5′-untranslated region. In all cases for which there is adequate information, the KRAB domain is found at the NH2-terminus of the respective protein. In one zinc-finger-encoding cDNA clone that we characterized further in this work, BRc1744 (ZNF45), the KRAB domain most probably constitutes the entire second exon of the gene. Based on the data, it is tempting to speculate that the FAX- and KRAB-containing zinc finger genes define subfamilies of genes with overlapping functions that participate in the regulation of common or similar developmental programs.",
author = "Consiantinou-Deltas, {C. D.} and John Gilbert and Bartlett, {R. J.} and M. Herbstreith and Roses, {A. D.} and Lee, {J. E.}",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/0888-7543(92)90451-W",
language = "English",
volume = "12",
pages = "581--589",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The identification and characterization of KRAB-domain-containing zinc finger proteins

AU - Consiantinou-Deltas, C. D.

AU - Gilbert, John

AU - Bartlett, R. J.

AU - Herbstreith, M.

AU - Roses, A. D.

AU - Lee, J. E.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The zinc finger motif is a highly conserved tandemly repeated sequence of 28-30 amino acids that was first identified in transcription factor TFIIIA from Xenopus laevis. Subsequently, similar motifs were found and characterized in many genes from mammalian genomes and the genomes of lower eukaryotes such as Drosophila and yeast, thereby defining a large superfamily of genes. Non-finger-coding modules conserved among members of subfamilies of zinc finger genes have been described in the murine genome (finger-associated boxes, or FAX domain) and the human genome (Krüppel-associated boxes, or KRAB domain). Here we report the identification and partial characterization of more members of the human KRAB-containing subfamily of genes. Based on Southern blot hybridization experiments, they also are zinc-finger-coding genes. All members share a highly homologous 42-amino-acid-long a element of the described KRAB domain. The conservation extends to the murine developmentally expressed zinc finger gene, mKr2. The homologous sequences, however, are part of the 5′-untranslated region. In all cases for which there is adequate information, the KRAB domain is found at the NH2-terminus of the respective protein. In one zinc-finger-encoding cDNA clone that we characterized further in this work, BRc1744 (ZNF45), the KRAB domain most probably constitutes the entire second exon of the gene. Based on the data, it is tempting to speculate that the FAX- and KRAB-containing zinc finger genes define subfamilies of genes with overlapping functions that participate in the regulation of common or similar developmental programs.

AB - The zinc finger motif is a highly conserved tandemly repeated sequence of 28-30 amino acids that was first identified in transcription factor TFIIIA from Xenopus laevis. Subsequently, similar motifs were found and characterized in many genes from mammalian genomes and the genomes of lower eukaryotes such as Drosophila and yeast, thereby defining a large superfamily of genes. Non-finger-coding modules conserved among members of subfamilies of zinc finger genes have been described in the murine genome (finger-associated boxes, or FAX domain) and the human genome (Krüppel-associated boxes, or KRAB domain). Here we report the identification and partial characterization of more members of the human KRAB-containing subfamily of genes. Based on Southern blot hybridization experiments, they also are zinc-finger-coding genes. All members share a highly homologous 42-amino-acid-long a element of the described KRAB domain. The conservation extends to the murine developmentally expressed zinc finger gene, mKr2. The homologous sequences, however, are part of the 5′-untranslated region. In all cases for which there is adequate information, the KRAB domain is found at the NH2-terminus of the respective protein. In one zinc-finger-encoding cDNA clone that we characterized further in this work, BRc1744 (ZNF45), the KRAB domain most probably constitutes the entire second exon of the gene. Based on the data, it is tempting to speculate that the FAX- and KRAB-containing zinc finger genes define subfamilies of genes with overlapping functions that participate in the regulation of common or similar developmental programs.

UR - http://www.scopus.com/inward/record.url?scp=0026600575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026600575&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(92)90451-W

DO - 10.1016/0888-7543(92)90451-W

M3 - Article

C2 - 1559709

AN - SCOPUS:0026600575

VL - 12

SP - 581

EP - 589

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -