The human REV1 gene codes for a DNA template-dependent dCMP transferase

Wensheng Lin, Hua Xin, Yanbin Zhang, Xiaohua Wu, Fenghua Yuan, Zhigang Wang

Research output: Contribution to journalArticle

153 Scopus citations


DNA is frequently damaged by various physical and chemical agents, DNA damage can lead to mutations during replication. In the yeast Saccharomyces cerevisiae, the damage-induced mutagenesis pathway requires the Rev1 protein. We have isolated a human cDNA homologous to the yeast REV1 gene. The human REV1 cDNA consists of 4255 bp and codes for a protein of 1251 amino acid residues with a calculated molecular weight of 138 248 Da. The human REV1 gene is localized between 2q11.1 and 2q11.2. We show that the human REV1 protein is a dCMP transferase that specifically inserts a dCMP residue opposite a DNA template G. In addition, the human REV1 transferase is able to efficiently and specifically insert a dCMP opposite a DNA template apurinic/apyrimidinic (AP) site or a uracil residue. These results suggest that the REV1 transferase may play a critical role during mutagenic translesion DNA synthesis bypassing a template AP site in human cells. Consistent with its role as a fundamental mutagenic protein, the REV1 gene is ubiquitously expressed in various human tissues.

Original languageEnglish (US)
Pages (from-to)4468-4475
Number of pages8
JournalNucleic acids research
Issue number22
StatePublished - Nov 15 1999

ASJC Scopus subject areas

  • Genetics

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