The human RAD18 gene product interacts with HHR6A and HHR6B

H. Xin, W. Lin, W. Sumanasekera, Yanbin Zhang, X. Wu, Z. Wang

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

During DNA replication, lesion bypass is an important cellular response to unrepaired damage in the genome. In the yeast Saccharomyces cerevisiae, Rad6 and Rad18 are required for both the error-free and error-prone lesion bypass mechanisms. Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. Two closely related human homologs of yeast Rad6 have been identified as HHR6A and HHR6B. Here,we report a full-lenght cDNA coding for the human homolog of yeast RAD18 The human RAD18 gene codes for a protein of 484 amino acid residues with a calculated molecular weight of 54 804 Da, and the gene is localized to chromosome 3 between reference intervals D3S3591 and D3S1283. Human RAD18 protien (hRAD18) was found to interact with HHR6A and HHR6B. When co-expressed in yeast cells, stable hRAD18-HHR6A and hRAD18-HHR6B protien complexes were identified and purified to near homogenity. Thus, through interaction and complex formation with HHR6A and HHR6B, RAD18 protein may play an important role in lesion bypass mechanisms in humans. Consistent with its role as a fundamental lesion bypass protein, the RAD18 gene is ubiquitously expressed in various human tissues.

Original languageEnglish
Pages (from-to)2847-2854
Number of pages8
JournalNucleic Acids Research
Volume28
Issue number14
StatePublished - Jul 15 2000
Externally publishedYes

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Yeasts
Genes
Proteins
Chromosomes, Human, Pair 3
DNA Replication
Saccharomyces cerevisiae
Complementary DNA
Molecular Weight
Genome
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Xin, H., Lin, W., Sumanasekera, W., Zhang, Y., Wu, X., & Wang, Z. (2000). The human RAD18 gene product interacts with HHR6A and HHR6B. Nucleic Acids Research, 28(14), 2847-2854.

The human RAD18 gene product interacts with HHR6A and HHR6B. / Xin, H.; Lin, W.; Sumanasekera, W.; Zhang, Yanbin; Wu, X.; Wang, Z.

In: Nucleic Acids Research, Vol. 28, No. 14, 15.07.2000, p. 2847-2854.

Research output: Contribution to journalArticle

Xin, H, Lin, W, Sumanasekera, W, Zhang, Y, Wu, X & Wang, Z 2000, 'The human RAD18 gene product interacts with HHR6A and HHR6B', Nucleic Acids Research, vol. 28, no. 14, pp. 2847-2854.
Xin H, Lin W, Sumanasekera W, Zhang Y, Wu X, Wang Z. The human RAD18 gene product interacts with HHR6A and HHR6B. Nucleic Acids Research. 2000 Jul 15;28(14):2847-2854.
Xin, H. ; Lin, W. ; Sumanasekera, W. ; Zhang, Yanbin ; Wu, X. ; Wang, Z. / The human RAD18 gene product interacts with HHR6A and HHR6B. In: Nucleic Acids Research. 2000 ; Vol. 28, No. 14. pp. 2847-2854.
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