The human λ L chain Ig locus: Recharacterization of JCλ6 and identification of a functional JCλ7

Thomas R. Bauer, Bonnie B Blomberg

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The human λ L chain Ig gene complex consists of multiple JC gene segments. A seventh human λ C region gene segment, Cλ7, was found 2.7 kb downstream of Cλ6 in this gene complex. A J λ gene segment, Jλ7, was found 1.2 kb upstream of Cλ7 and contains potentially functional nonamer and heptamer recombination sites, an RNA splice site and J coding region. Cλ7 maintains an open reading frame and encodes a new λ isotype. Cλ7 encodes Kern+ and Oz- determinants, but does not encode any of the Kern+Oz- myeloma proteins published to date. Nevertheless, we present evidence that JCλ7 is transcribed in normal lymphocytes and is functional. In contrast, we present new data that the Cλ6 gene segment, reported by others to encode the Kern+Oz- protein, is non-functional due to a 4-bp insertion in our cosmid clone. The 4-bp insertion was characterized further in 32 genomic DNA samples by producing a distinctive restriction fragment length and verified by the DNA sequences of the polymerase chain reaction products of two different cell lines. We discuss the possibility that the Kern+Oz- myeloma proteins do not define an isotype and are not encoded by JCλ7 nor other non-allelic genes, and we discuss the level of expression of JCλ7 as compared to that of JCλ2 and JCλ3.

Original languageEnglish
Pages (from-to)2813-2820
Number of pages8
JournalJournal of Immunology
Volume146
Issue number8
StatePublished - Apr 15 1991

Fingerprint

Genes
Myeloma Proteins
Cosmids
Immunoglobulin Genes
RNA Splice Sites
DNA-Directed DNA Polymerase
Genetic Recombination
Open Reading Frames
Clone Cells
Lymphocytes
Cell Line
Polymerase Chain Reaction
DNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

The human λ L chain Ig locus : Recharacterization of JCλ6 and identification of a functional JCλ7. / Bauer, Thomas R.; Blomberg, Bonnie B.

In: Journal of Immunology, Vol. 146, No. 8, 15.04.1991, p. 2813-2820.

Research output: Contribution to journalArticle

@article{b4aba86fe4954b529eeaa31cd82d5266,
title = "The human λ L chain Ig locus: Recharacterization of JCλ6 and identification of a functional JCλ7",
abstract = "The human λ L chain Ig gene complex consists of multiple JC gene segments. A seventh human λ C region gene segment, Cλ7, was found 2.7 kb downstream of Cλ6 in this gene complex. A J λ gene segment, Jλ7, was found 1.2 kb upstream of Cλ7 and contains potentially functional nonamer and heptamer recombination sites, an RNA splice site and J coding region. Cλ7 maintains an open reading frame and encodes a new λ isotype. Cλ7 encodes Kern+ and Oz- determinants, but does not encode any of the Kern+Oz- myeloma proteins published to date. Nevertheless, we present evidence that JCλ7 is transcribed in normal lymphocytes and is functional. In contrast, we present new data that the Cλ6 gene segment, reported by others to encode the Kern+Oz- protein, is non-functional due to a 4-bp insertion in our cosmid clone. The 4-bp insertion was characterized further in 32 genomic DNA samples by producing a distinctive restriction fragment length and verified by the DNA sequences of the polymerase chain reaction products of two different cell lines. We discuss the possibility that the Kern+Oz- myeloma proteins do not define an isotype and are not encoded by JCλ7 nor other non-allelic genes, and we discuss the level of expression of JCλ7 as compared to that of JCλ2 and JCλ3.",
author = "Bauer, {Thomas R.} and Blomberg, {Bonnie B}",
year = "1991",
month = "4",
day = "15",
language = "English",
volume = "146",
pages = "2813--2820",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - The human λ L chain Ig locus

T2 - Recharacterization of JCλ6 and identification of a functional JCλ7

AU - Bauer, Thomas R.

AU - Blomberg, Bonnie B

PY - 1991/4/15

Y1 - 1991/4/15

N2 - The human λ L chain Ig gene complex consists of multiple JC gene segments. A seventh human λ C region gene segment, Cλ7, was found 2.7 kb downstream of Cλ6 in this gene complex. A J λ gene segment, Jλ7, was found 1.2 kb upstream of Cλ7 and contains potentially functional nonamer and heptamer recombination sites, an RNA splice site and J coding region. Cλ7 maintains an open reading frame and encodes a new λ isotype. Cλ7 encodes Kern+ and Oz- determinants, but does not encode any of the Kern+Oz- myeloma proteins published to date. Nevertheless, we present evidence that JCλ7 is transcribed in normal lymphocytes and is functional. In contrast, we present new data that the Cλ6 gene segment, reported by others to encode the Kern+Oz- protein, is non-functional due to a 4-bp insertion in our cosmid clone. The 4-bp insertion was characterized further in 32 genomic DNA samples by producing a distinctive restriction fragment length and verified by the DNA sequences of the polymerase chain reaction products of two different cell lines. We discuss the possibility that the Kern+Oz- myeloma proteins do not define an isotype and are not encoded by JCλ7 nor other non-allelic genes, and we discuss the level of expression of JCλ7 as compared to that of JCλ2 and JCλ3.

AB - The human λ L chain Ig gene complex consists of multiple JC gene segments. A seventh human λ C region gene segment, Cλ7, was found 2.7 kb downstream of Cλ6 in this gene complex. A J λ gene segment, Jλ7, was found 1.2 kb upstream of Cλ7 and contains potentially functional nonamer and heptamer recombination sites, an RNA splice site and J coding region. Cλ7 maintains an open reading frame and encodes a new λ isotype. Cλ7 encodes Kern+ and Oz- determinants, but does not encode any of the Kern+Oz- myeloma proteins published to date. Nevertheless, we present evidence that JCλ7 is transcribed in normal lymphocytes and is functional. In contrast, we present new data that the Cλ6 gene segment, reported by others to encode the Kern+Oz- protein, is non-functional due to a 4-bp insertion in our cosmid clone. The 4-bp insertion was characterized further in 32 genomic DNA samples by producing a distinctive restriction fragment length and verified by the DNA sequences of the polymerase chain reaction products of two different cell lines. We discuss the possibility that the Kern+Oz- myeloma proteins do not define an isotype and are not encoded by JCλ7 nor other non-allelic genes, and we discuss the level of expression of JCλ7 as compared to that of JCλ2 and JCλ3.

UR - http://www.scopus.com/inward/record.url?scp=0025849763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025849763&partnerID=8YFLogxK

M3 - Article

C2 - 1901886

AN - SCOPUS:0025849763

VL - 146

SP - 2813

EP - 2820

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -