The histone variant macroH2A is an epigenetic regulator of key developmental genes

Marcus Buschbeck, Iris Uribesalgo, Indra Wibowo, Pau Rué, David Martin, Arantxa Gutierrez, LLuis Morey, Roderic Guigó, Hernán López-Schier, Luciano Di Croce

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The histone variants macroH2A1 and macroH2A2 are associated with X chromosome inactivation in female mammals. However, the physiological function of macroH2A proteins on autosomes is poorly understood. Microarray-based analysis in human male pluripotent cells uncovered occupancy of both macroH2A variants at many genes encoding key regulators of development and cell fate decisions. On these genes, the presence of macroH2A1+2 is a repressive mark that overlaps locally and functionally with Polycomb repressive complex 2. We demonstrate that macroH2A1+2 contribute to the fine-tuning of temporal activation of HOXA cluster genes during neuronal differentiation. Furthermore, elimination of macroH2A2 function in zebrafish embryos produced severe but specific phenotypes. Taken together, our data demonstrate that macroH2A variants constitute an important epigenetic mark involved in the concerted regulation of gene expression programs during cellular differentiation and vertebrate development.

Original languageEnglish (US)
Pages (from-to)1074-1079
Number of pages6
JournalNature Structural and Molecular Biology
Volume16
Issue number10
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

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Developmental Genes
Epigenomics
Polycomb Repressive Complex 2
X Chromosome Inactivation
Gene Expression Regulation
Zebrafish
Microarray Analysis
Multigene Family
Histones
Genes
Vertebrates
Mammals
Embryonic Structures
Phenotype
Proteins
macroH2A histone

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Buschbeck, M., Uribesalgo, I., Wibowo, I., Rué, P., Martin, D., Gutierrez, A., ... Di Croce, L. (2009). The histone variant macroH2A is an epigenetic regulator of key developmental genes. Nature Structural and Molecular Biology, 16(10), 1074-1079. https://doi.org/10.1038/nsmb.1665

The histone variant macroH2A is an epigenetic regulator of key developmental genes. / Buschbeck, Marcus; Uribesalgo, Iris; Wibowo, Indra; Rué, Pau; Martin, David; Gutierrez, Arantxa; Morey, LLuis; Guigó, Roderic; López-Schier, Hernán; Di Croce, Luciano.

In: Nature Structural and Molecular Biology, Vol. 16, No. 10, 01.10.2009, p. 1074-1079.

Research output: Contribution to journalArticle

Buschbeck, M, Uribesalgo, I, Wibowo, I, Rué, P, Martin, D, Gutierrez, A, Morey, LL, Guigó, R, López-Schier, H & Di Croce, L 2009, 'The histone variant macroH2A is an epigenetic regulator of key developmental genes', Nature Structural and Molecular Biology, vol. 16, no. 10, pp. 1074-1079. https://doi.org/10.1038/nsmb.1665
Buschbeck M, Uribesalgo I, Wibowo I, Rué P, Martin D, Gutierrez A et al. The histone variant macroH2A is an epigenetic regulator of key developmental genes. Nature Structural and Molecular Biology. 2009 Oct 1;16(10):1074-1079. https://doi.org/10.1038/nsmb.1665
Buschbeck, Marcus ; Uribesalgo, Iris ; Wibowo, Indra ; Rué, Pau ; Martin, David ; Gutierrez, Arantxa ; Morey, LLuis ; Guigó, Roderic ; López-Schier, Hernán ; Di Croce, Luciano. / The histone variant macroH2A is an epigenetic regulator of key developmental genes. In: Nature Structural and Molecular Biology. 2009 ; Vol. 16, No. 10. pp. 1074-1079.
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