The histone deacetylase 9 gene encodes multiple protein isoforms

Kevin Petrie, Fabien Guidez, Louise Howell, Lyn Healy, Samuel Waxman, Mel Greaves, Arthur Zelent

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Histone deacetylases (HDACs) perform an important function in transcriptional regulation by modifying the core histones of the nucleosome. We have now fully characterized a new member of the Class II HDAC family, HDAC9. The enzyme contains a conserved deacetylase domain, represses reporter activity when recruited to a promoter, and utilizes histones H3 and H4 as substrates in vitro and in vivo. HDAC9 is expressed in a tissue-specific pattern that partially overlaps that of HDAC4. Within the human hematopoietic system, expression of HDAC9 is biased toward cells of monocytic and lymphoid lineages. The HDAC9 gene encodes multiple protein isoforms, some of which display distinct cellular localization patterns. For example, full-length HDAC9 is localized in the nucleus, but the isoform lacking the region encoded by exon 7 is in the cytoplasm. HDAC9 interacts and co-localizes in vivo with a number of transcriptional repressors and co-repressors, including TEL and N-CoR, whose functions have been implicated in the pathogenesis of hematological malignancies. These results suggest that HDAC9 plays a role in hematopoiesis; its deregulated expression may be associated with some human cancers.

Original languageEnglish (US)
Pages (from-to)16059-16072
Number of pages14
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOIs
StatePublished - May 2 2003

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Petrie, K., Guidez, F., Howell, L., Healy, L., Waxman, S., Greaves, M., & Zelent, A. (2003). The histone deacetylase 9 gene encodes multiple protein isoforms. Journal of Biological Chemistry, 278(18), 16059-16072. https://doi.org/10.1074/jbc.M212935200