The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells

V. C. Ogilvie, B. J. Wilson, S. M. Nicol, N. A. Morrice, L. R. Saunders, Glen N Barber, F. V. Fuller-Pace

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as heterodimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from ∼150 to ∼400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.

Original languageEnglish
Pages (from-to)1470-1480
Number of pages11
JournalNucleic Acids Research
Volume31
Issue number5
DOIs
StatePublished - Mar 1 2003

Fingerprint

DEAD-box RNA Helicases
Proteins
Messenger RNA
Two-Hybrid System Techniques
Interphase
Cell Nucleus
Immunoprecipitation
Gel Chromatography
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Ogilvie, V. C., Wilson, B. J., Nicol, S. M., Morrice, N. A., Saunders, L. R., Barber, G. N., & Fuller-Pace, F. V. (2003). The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells. Nucleic Acids Research, 31(5), 1470-1480. https://doi.org/10.1093/nar/gkg236

The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells. / Ogilvie, V. C.; Wilson, B. J.; Nicol, S. M.; Morrice, N. A.; Saunders, L. R.; Barber, Glen N; Fuller-Pace, F. V.

In: Nucleic Acids Research, Vol. 31, No. 5, 01.03.2003, p. 1470-1480.

Research output: Contribution to journalArticle

Ogilvie, VC, Wilson, BJ, Nicol, SM, Morrice, NA, Saunders, LR, Barber, GN & Fuller-Pace, FV 2003, 'The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells', Nucleic Acids Research, vol. 31, no. 5, pp. 1470-1480. https://doi.org/10.1093/nar/gkg236
Ogilvie, V. C. ; Wilson, B. J. ; Nicol, S. M. ; Morrice, N. A. ; Saunders, L. R. ; Barber, Glen N ; Fuller-Pace, F. V. / The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells. In: Nucleic Acids Research. 2003 ; Vol. 31, No. 5. pp. 1470-1480.
@article{719224c7067940899529f69b21a810d9,
title = "The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells",
abstract = "The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90{\%} identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as heterodimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from ∼150 to ∼400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.",
author = "Ogilvie, {V. C.} and Wilson, {B. J.} and Nicol, {S. M.} and Morrice, {N. A.} and Saunders, {L. R.} and Barber, {Glen N} and Fuller-Pace, {F. V.}",
year = "2003",
month = "3",
day = "1",
doi = "10.1093/nar/gkg236",
language = "English",
volume = "31",
pages = "1470--1480",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells

AU - Ogilvie, V. C.

AU - Wilson, B. J.

AU - Nicol, S. M.

AU - Morrice, N. A.

AU - Saunders, L. R.

AU - Barber, Glen N

AU - Fuller-Pace, F. V.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as heterodimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from ∼150 to ∼400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.

AB - The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as heterodimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from ∼150 to ∼400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.

UR - http://www.scopus.com/inward/record.url?scp=0037338455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037338455&partnerID=8YFLogxK

U2 - 10.1093/nar/gkg236

DO - 10.1093/nar/gkg236

M3 - Article

C2 - 12595555

AN - SCOPUS:0037338455

VL - 31

SP - 1470

EP - 1480

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 5

ER -