The generation of cytotoxic T lymphocytes against acetaldehyde-modified syngeneic cells

T. Hidetaka, Michael A Kolber

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The major metabolic product of ethanol is acetaldehyde. It is highly reactive with proteins. In situ this modification is significant enough to generate an antibody response. Whether an effector cellular immune response can be generated against these acetaldehyde modified adducts on syngeneic cells is not known. In this paper we have demonstrated in the murine system that acetaldehyde modified splenic cells can generate cytotoxic T lymphocytes (CTL). These CTL are specific for the acetaldehyde modified syngeneic cells, and not acetaldehyde modified allogeneic cells. The ability of the CTL to lyse specific targets is dependent on the formation of stable acetaldehyde adducts. Cold target inhibition studies reveal that modified syngeneic cells can inhibit lysis as effectively as unmodified cells. Therefore, the present study lends support to the hypothesis that acetaldehyde modified cells can generate a cellular immune response and may do so in pathologic states.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume14
Issue number6
DOIs
StatePublished - Dec 1 1990

Fingerprint

T-cells
Acetaldehyde
Cytotoxic T-Lymphocytes
Cellular Immunity
Antibody Formation
Ethanol
Antibodies

Keywords

  • Acetaldehyde
  • Alcohol
  • CTL
  • Ethanol

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

The generation of cytotoxic T lymphocytes against acetaldehyde-modified syngeneic cells. / Hidetaka, T.; Kolber, Michael A.

In: Alcoholism: Clinical and Experimental Research, Vol. 14, No. 6, 01.12.1990, p. 893-899.

Research output: Contribution to journalArticle

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