The gene for the longest known Escherichia coli protein is a member of helicase superfamily II

N. B. Reuven, E. V. Koonin, K. E. Rudd, Murray P Deutscher

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The Escherichia coli rnt gene, which encodes the RNA-processing enzyme RNase T, is cotranscribed with a downstream gene. Complete sequencing of this gene indicates that its coding region encompasses 1,538 amino acids, making it the longest known protein in E. coli. The gene (tentatively termed lhr for long helicase related) contains the seven conserved motifs of the DNA and RNA helicase superfamily II. An ~170-kDa protein is observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 35S-labeled extracts prepared from cells in which lhr is under the control of an induced T7 promoter. This protein is absent when lhr is interrupted or when no plasmid is present. Downstream of lhr is the C-terminal region of a convergent gene with homology to glutaredoxin. Interruptions of chromosomal lhr at two different positions within the gene do not affect the growth of E. coli at various temperatures in rich or minimal medium, indicating that lhr is not essential for usual laboratory growth. lhr interruption also has no effect on anaerobic growth. In addition, cells lacking Lhr recover normally from starvation, plate phage normally, and display normal sensitivities to UV irradiation and H2O2. Southern analysis showed that no other gene closely related to lhr is present on the E. coli chromosome. These data expand the known size range of E. coli proteins and suggest that very large helicases are present in this organism.

Original languageEnglish
Pages (from-to)5393-5400
Number of pages8
JournalJournal of Bacteriology
Volume177
Issue number19
StatePublished - Dec 1 1995

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Escherichia coli Proteins
Genes
Escherichia coli
Growth
Glutaredoxins
RNA Helicases
DNA Helicases
Gene Order
Starvation
Cell Extracts
Sodium Dodecyl Sulfate
Bacteriophages
Polyacrylamide Gel Electrophoresis
Proteins
Plasmids
Chromosomes
RNA
Amino Acids
Temperature
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Reuven, N. B., Koonin, E. V., Rudd, K. E., & Deutscher, M. P. (1995). The gene for the longest known Escherichia coli protein is a member of helicase superfamily II. Journal of Bacteriology, 177(19), 5393-5400.

The gene for the longest known Escherichia coli protein is a member of helicase superfamily II. / Reuven, N. B.; Koonin, E. V.; Rudd, K. E.; Deutscher, Murray P.

In: Journal of Bacteriology, Vol. 177, No. 19, 01.12.1995, p. 5393-5400.

Research output: Contribution to journalArticle

Reuven, NB, Koonin, EV, Rudd, KE & Deutscher, MP 1995, 'The gene for the longest known Escherichia coli protein is a member of helicase superfamily II', Journal of Bacteriology, vol. 177, no. 19, pp. 5393-5400.
Reuven NB, Koonin EV, Rudd KE, Deutscher MP. The gene for the longest known Escherichia coli protein is a member of helicase superfamily II. Journal of Bacteriology. 1995 Dec 1;177(19):5393-5400.
Reuven, N. B. ; Koonin, E. V. ; Rudd, K. E. ; Deutscher, Murray P. / The gene for the longest known Escherichia coli protein is a member of helicase superfamily II. In: Journal of Bacteriology. 1995 ; Vol. 177, No. 19. pp. 5393-5400.
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