The fractionation of nitrogen and oxygen isotopes in macroalgae during the assimilation of nitrate

Peter K Swart, S. Evans, T. Capo, M. A. Altabet

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

In order to determine and understand the stable isotope fractionation of 18O and 15N manifested during assimilation of NO3 - in marine macro-benthic algae, two species (Ulva sp. and Agardhiella sp.) have been grown in a wide range of NO3 - concentrations (2-500 μM). Two types of experiments were performed. The first was one in which the concentration of the NO3 - was allowed to drift downward as it was assimilated by the algae, between 24 hour replacements of media. These experiments proceeded for periods of between 7 and 10 days. A second set of experiments maintained the NO3 - concentration at a low steady-state value by means of a syringe pump. The effective fractionation during the assimilation of the NO3 - was determined by measuring the δ15N of both the (i) new algal growth and (ii) residual NO3 - in the free-drift experiments after 0, 12, 24 and 48 h. Modelling these data show that the fractionation during assimilation is dependent upon the concentration of NO3 - and is effectively 0 at concentrations of less than ∼2 μM. The change in the fractionation with respect to concentration is the greatest at lower concentrations (2-10 μM). The fractionation stablizes between 4 and 6‰ at concentrations of between 50 and 500 μM. Although the δ18O and δ15N values of NO3 - in the residual solution were correlated, the slope of relationship also varied with respect to NO3 - concentration, with slopes of greater than unity at low concentration. These results suggest shifts in the dominant fractionation mechanism of 15N and 18O between concentrations of 1 and 10 μM NO3 -. At higher NO3 - concentrations (>10-50 μM), fractionation during assimilation will lead to δ15N values in algal biomass lower than the ambient NO3 - and 15N enrichments in the residual NO3 -.

Original languageEnglish (US)
Pages (from-to)6147-6157
Number of pages11
JournalBiogeosciences
Volume11
Issue number21
DOIs
StatePublished - Nov 13 2014

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Earth-Surface Processes

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