The expression signature of in vitro senescence resembles mouse but not human aging

Kristian Wennmalm, Claes R Wahlestedt, Ola Larsson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: The biological mechanisms that underlie aging have not yet been fully identified. Senescence, a phenomenon occurring in vitro, limits the number of cell divisions in mammalian cell cultures and has been suggested to contribute to aging. Results: We investigated whether the changes in gene expression that occur during mammalian aging and induction of cellular senescence are similar. We compared changes of gene expression in seven microarray datasets from aging human, mouse and rat, as well as four microarray clatasets from senescent cells of man and mouse. The clatasets were publicly available or obtained from other laboratories. Correlation measures were used to establish similarities of the expression profiles and gene ontology analyses to identify functional groups of genes that are co-regulated. Robust similarities were established between aging in different species and tissues, indicating that there is an aging transcriptome. Although some cross-species comparisons displayed high correlation, intra-species similarities were more reliable. Similarly, a senescence transcriptome was demonstrated that is conserved across cell types. A similarity between the expression signatures of cellular senescence and aging could be established in mouse, but not in human. Conclusion: Our study is the first to use microarray data from several studies and laboratories for dissection of a complex biological phenotype. We demonstrate the presence of a mammalian aging transcriptome, and discuss why similarity between cellular senescence and aging is apparent in aging mice only.

Original languageEnglish
Article numberR109
JournalGenome Biology
Volume6
Issue number13
DOIs
StatePublished - Dec 16 2005
Externally publishedYes

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Cell Aging
senescence
transcriptome
Transcriptome
mice
gene expression
Gene Expression
Gene Ontology
cell division
cell culture
genes
Cell Division
cells
Dissection
phenotype
Cell Culture Techniques
In Vitro Techniques
rats
Phenotype
gene

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

The expression signature of in vitro senescence resembles mouse but not human aging. / Wennmalm, Kristian; Wahlestedt, Claes R; Larsson, Ola.

In: Genome Biology, Vol. 6, No. 13, R109, 16.12.2005.

Research output: Contribution to journalArticle

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