The expression of CCL2 by T lymphocytes of mammary tumor bearers

Role of tumor-derived factors

Jennifer L. Owen, Diana M Lopez, Joseph F. Grosso, Kathleen M. Guthrie, Lynn M. Herbert, Marta Torroella-Kouri, Vijaya Iragavarapu-Charyulu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Tumor-associated chemokines, including CC chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2), are thought to play many roles in cancer progression. Here we demonstrate the novel finding that during growth of the D1-7,12-dimethylbenzanthracene-3 mammary tumor in BALB/c mice, there is a dramatic up-regulation of CCL2 in splenic T cells at both the mRNA and protein levels upon stimulation. Of particular relevance is the finding that tumor-infiltrating T cells also produce high levels of CCL2. While a variety of tumor cell lines have been found to produce CCL2, we found no detectable levels of CCL2 protein in supernatants of the cultured mammary tumor cells. Investigation of the mechanisms involved in CCL2 induction showed that treatment of splenic T cells with the tumor-derived factors GM-CSF and phosphatidyl serine (PS) resulted in increased CCL2 production. This increased production may be involved in the downregulation of IFN-γ by the T cells of tumor-bearing mice previously reported in this model, as treatment of splenic T lymphocytes with CCL2 resulted in a decreased secretion of IFN-γ by those cells.

Original languageEnglish
Pages (from-to)122-135
Number of pages14
JournalCellular Immunology
Volume235
Issue number2
DOIs
StatePublished - Jun 1 2005

Fingerprint

CC Chemokines
Chemokine CCL2
Breast Neoplasms
Ligands
T-Lymphocytes
Neoplasms
Cultured Tumor Cells
9,10-Dimethyl-1,2-benzanthracene
Phosphatidylserines
Granulocyte-Macrophage Colony-Stimulating Factor
Tumor Cell Line
Chemokines
Proteins
Up-Regulation
Down-Regulation
Messenger RNA
Growth

Keywords

  • CCL2
  • Chemokines
  • GM-CSF
  • IFN-γ
  • Mammary tumor
  • PS
  • T lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Owen, J. L., Lopez, D. M., Grosso, J. F., Guthrie, K. M., Herbert, L. M., Torroella-Kouri, M., & Iragavarapu-Charyulu, V. (2005). The expression of CCL2 by T lymphocytes of mammary tumor bearers: Role of tumor-derived factors. Cellular Immunology, 235(2), 122-135. https://doi.org/10.1016/j.cellimm.2005.08.032

The expression of CCL2 by T lymphocytes of mammary tumor bearers : Role of tumor-derived factors. / Owen, Jennifer L.; Lopez, Diana M; Grosso, Joseph F.; Guthrie, Kathleen M.; Herbert, Lynn M.; Torroella-Kouri, Marta; Iragavarapu-Charyulu, Vijaya.

In: Cellular Immunology, Vol. 235, No. 2, 01.06.2005, p. 122-135.

Research output: Contribution to journalArticle

Owen, JL, Lopez, DM, Grosso, JF, Guthrie, KM, Herbert, LM, Torroella-Kouri, M & Iragavarapu-Charyulu, V 2005, 'The expression of CCL2 by T lymphocytes of mammary tumor bearers: Role of tumor-derived factors', Cellular Immunology, vol. 235, no. 2, pp. 122-135. https://doi.org/10.1016/j.cellimm.2005.08.032
Owen, Jennifer L. ; Lopez, Diana M ; Grosso, Joseph F. ; Guthrie, Kathleen M. ; Herbert, Lynn M. ; Torroella-Kouri, Marta ; Iragavarapu-Charyulu, Vijaya. / The expression of CCL2 by T lymphocytes of mammary tumor bearers : Role of tumor-derived factors. In: Cellular Immunology. 2005 ; Vol. 235, No. 2. pp. 122-135.
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