The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure

Liuqin Zhu; Murray P. Deutscher

doi:10.1016/0378-1119(92)90072-W

The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure

Liuqin Zhu, Murray P. Deutscher

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article

8 Scopus citations

Abstract

A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

Original language	English (US)
Pages (from-to)	101-106
Number of pages	6
Journal	Gene
Volume	119
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90072-W
State	Published - Sep 21 1992

Keywords

Rho-independent termination site
RNA degradation
transcriptional coupling

ASJC Scopus subject areas

Genetics

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Zhu, L., & Deutscher, M. P. (1992). The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure. Gene, 119(1), 101-106. https://doi.org/10.1016/0378-1119(92)90072-W

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T1 - The Escherichia coli rna gene encoding RNase I

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AU - Deutscher, Murray P.

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AB - A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

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