TY - JOUR
T1 - The Escherichia coli rna gene encoding RNase I
T2 - sequence and unusual promoter structure
AU - Zhu, Liuqin
AU - Deutscher, Murray P.
N1 - Funding Information:
This work was supported by grant GM16317 from the National Institutes of Health. We thank Nina Bather Reuven for assistance with some of these experiments.
PY - 1992/9/21
Y1 - 1992/9/21
N2 - A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.
AB - A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.
KW - Rho-independent termination site
KW - RNA degradation
KW - transcriptional coupling
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U2 - 10.1016/0378-1119(92)90072-W
DO - 10.1016/0378-1119(92)90072-W
M3 - Article
C2 - 1398076
AN - SCOPUS:0026667856
VL - 119
SP - 101
EP - 106
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -