The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure

Liuqin Zhu, Murray P. Deutscher

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalGene
Volume119
Issue number1
DOIs
StatePublished - Sep 21 1992

Keywords

  • Rho-independent termination site
  • RNA degradation
  • transcriptional coupling

ASJC Scopus subject areas

  • Genetics

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