The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure

Liuqin Zhu; Murray P. Deutscher

doi:10.1016/0378-1119(92)90072-W

The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure

Liuqin Zhu, Murray P. Deutscher

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

Original language	English (US)
Pages (from-to)	101-106
Number of pages	6
Journal	Gene
Volume	119
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90072-W
State	Published - Sep 21 1992

Keywords

Rho-independent termination site
RNA degradation
transcriptional coupling

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(92)90072-W

Fingerprint Dive into the research topics of 'The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure'. Together they form a unique fingerprint.

Cite this

@article{e7cd128b757e474b9fcc2d98ad9e0e59,

title = "The Escherichia coli rna gene encoding RNase I: sequence and unusual promoter structure",

abstract = "A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.",

keywords = "Rho-independent termination site, RNA degradation, transcriptional coupling",

author = "Liuqin Zhu and Deutscher, {Murray P.}",

year = "1992",

month = sep,

day = "21",

doi = "10.1016/0378-1119(92)90072-W",

language = "English (US)",

volume = "119",

pages = "101--106",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - The Escherichia coli rna gene encoding RNase I

T2 - sequence and unusual promoter structure

AU - Zhu, Liuqin

AU - Deutscher, Murray P.

PY - 1992/9/21

Y1 - 1992/9/21

N2 - A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

AB - A clone containing the Escherichia coli rna gene encoding the nonspecific endoribonuclease, RNase I, was isolated and sequenced. The sequence of the 1070-nucleotide (nt) fragment agreed completely with that of a rna clone recently reported by Meador and Kennell [Gene 95 (1990) 1-7]. The transcription start point (tsp) of rna was identified using primer extension analysis, and its promoter sequence was established by comparison of RNase I expression levels in various deletion mutants. Our results indicate that the rna promoter is highly unusual. Its -35 region shows a poor match to the consensus sequence, and moreover, it is located within a stem-loop structure that apparently is a Rho-independent transcription termination site for an upstream gene.

KW - Rho-independent termination site

KW - RNA degradation

KW - transcriptional coupling

UR - http://www.scopus.com/inward/record.url?scp=0026667856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026667856&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(92)90072-W

DO - 10.1016/0378-1119(92)90072-W

M3 - Article

C2 - 1398076

AN - SCOPUS:0026667856

VL - 119

SP - 101

EP - 106

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -