The enzymic synthesis and degradation of starch. Part XI. Isophosphorylase

Isophosphorylase, isolated from the potato, and claimed by Bernfeld and Meutémédian to be responsible for the reversible formation of α-1:6-glycosidic linkages in amylopectin, has been prepared by the prescribed technique, and the key experiments of the above authors repeated. Although this repetition revealed an apparent agreement in some of the experimental data, we are forced to the conclusion that these experimental results have been misinterpreted by Bernfeld and Meutémédian and that an enzyme with the properties ascribed to "isophosphorylase" does not exist in the potato.