The effects of simultaneous antegrade/retrograde cardioplegia on cellular volumes and energy metabolism

Gang Li, Weichen Tian, Jian Wang, Bo Xiang, Lei Wang, Jixian Deng, Tomas Salerno, Roxanne Deslauriers, Ganghong Tian

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background and aim of the study: Simultaneous antegrade/retrograde cardioplegia (SARC) has been employed frequently during cardiac surgery to preserve the jeopardized myocardium. However, retrograde perfusion of SARC may interfere with myocardial drainage and disrupt myocardial fluid homeostasis, which may affect the myocardial energy metabolism and contractile function. The study was, therefore, designed to assess the effects of SARC on myocardial fluid homeostasis, cellular volumes, and energy metabolism. Methods: Eight isolated pig hearts were subjected to a protocol consisting of a 20-minute control perfusion, 120-minute SARC, and 20-minute reperfusion. The myocardial water content was monitored using near-infrared spectroscopy. Phosphorus-31 magnetic resonance (31P MR) spectroscopy was used to monitor the volumes of both intracellular and extracellular compartments and assess myocardial energy metabolism. Results: The near-infrared spectra showed that the 120-min SARC resulted in a 60 ± 12% increase in the myocardial water content. 31P MR spectra showed a 36 ± 4% increase in the intracellular compartment and a 54 ± 8% increase in the extracellular compartment during SARC relative to their initial volumes measured during control perfusion (100%). However, the myocardial energy metabolites (adenosine triphosphate [ATP] and phosphocreatine [PCr]) remained unchanged during the 120-minute SARC. Moreover, during reperfusion, the hearts showed an almost complete recovery in the left ventricular-developed pressure. Conclusions: A prolonged SARC resulted in water accumulation in both extracellular and intracellular compartments in the normal myocardium. Although its detrimental effect on tissue fluid homeostasis did not jeopardize the myocardial energy metabolism, a prolonged use of SARC should be avoided, particularly in the diseased hearts.

Original languageEnglish
Pages (from-to)437-443
Number of pages7
JournalJournal of Cardiac Surgery
Volume23
Issue number5
DOIs
StatePublished - Oct 1 2008

Fingerprint

Induced Heart Arrest
Energy Metabolism
Homeostasis
Perfusion
Reperfusion
Water
Myocardium
Magnetic Resonance Spectroscopy
Near-Infrared Spectroscopy
Phosphocreatine
Ventricular Pressure
Phosphorus
Thoracic Surgery
Drainage
Heart Diseases
Swine
Adenosine Triphosphate

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

The effects of simultaneous antegrade/retrograde cardioplegia on cellular volumes and energy metabolism. / Li, Gang; Tian, Weichen; Wang, Jian; Xiang, Bo; Wang, Lei; Deng, Jixian; Salerno, Tomas; Deslauriers, Roxanne; Tian, Ganghong.

In: Journal of Cardiac Surgery, Vol. 23, No. 5, 01.10.2008, p. 437-443.

Research output: Contribution to journalArticle

Li, Gang ; Tian, Weichen ; Wang, Jian ; Xiang, Bo ; Wang, Lei ; Deng, Jixian ; Salerno, Tomas ; Deslauriers, Roxanne ; Tian, Ganghong. / The effects of simultaneous antegrade/retrograde cardioplegia on cellular volumes and energy metabolism. In: Journal of Cardiac Surgery. 2008 ; Vol. 23, No. 5. pp. 437-443.
@article{cdd47d2c14ef47d98854841f074b9228,
title = "The effects of simultaneous antegrade/retrograde cardioplegia on cellular volumes and energy metabolism",
abstract = "Background and aim of the study: Simultaneous antegrade/retrograde cardioplegia (SARC) has been employed frequently during cardiac surgery to preserve the jeopardized myocardium. However, retrograde perfusion of SARC may interfere with myocardial drainage and disrupt myocardial fluid homeostasis, which may affect the myocardial energy metabolism and contractile function. The study was, therefore, designed to assess the effects of SARC on myocardial fluid homeostasis, cellular volumes, and energy metabolism. Methods: Eight isolated pig hearts were subjected to a protocol consisting of a 20-minute control perfusion, 120-minute SARC, and 20-minute reperfusion. The myocardial water content was monitored using near-infrared spectroscopy. Phosphorus-31 magnetic resonance (31P MR) spectroscopy was used to monitor the volumes of both intracellular and extracellular compartments and assess myocardial energy metabolism. Results: The near-infrared spectra showed that the 120-min SARC resulted in a 60 ± 12{\%} increase in the myocardial water content. 31P MR spectra showed a 36 ± 4{\%} increase in the intracellular compartment and a 54 ± 8{\%} increase in the extracellular compartment during SARC relative to their initial volumes measured during control perfusion (100{\%}). However, the myocardial energy metabolites (adenosine triphosphate [ATP] and phosphocreatine [PCr]) remained unchanged during the 120-minute SARC. Moreover, during reperfusion, the hearts showed an almost complete recovery in the left ventricular-developed pressure. Conclusions: A prolonged SARC resulted in water accumulation in both extracellular and intracellular compartments in the normal myocardium. Although its detrimental effect on tissue fluid homeostasis did not jeopardize the myocardial energy metabolism, a prolonged use of SARC should be avoided, particularly in the diseased hearts.",
author = "Gang Li and Weichen Tian and Jian Wang and Bo Xiang and Lei Wang and Jixian Deng and Tomas Salerno and Roxanne Deslauriers and Ganghong Tian",
year = "2008",
month = "10",
day = "1",
doi = "10.1111/j.1540-8191.2008.00690.x",
language = "English",
volume = "23",
pages = "437--443",
journal = "Journal of Cardiac Surgery",
issn = "0886-0440",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - The effects of simultaneous antegrade/retrograde cardioplegia on cellular volumes and energy metabolism

AU - Li, Gang

AU - Tian, Weichen

AU - Wang, Jian

AU - Xiang, Bo

AU - Wang, Lei

AU - Deng, Jixian

AU - Salerno, Tomas

AU - Deslauriers, Roxanne

AU - Tian, Ganghong

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Background and aim of the study: Simultaneous antegrade/retrograde cardioplegia (SARC) has been employed frequently during cardiac surgery to preserve the jeopardized myocardium. However, retrograde perfusion of SARC may interfere with myocardial drainage and disrupt myocardial fluid homeostasis, which may affect the myocardial energy metabolism and contractile function. The study was, therefore, designed to assess the effects of SARC on myocardial fluid homeostasis, cellular volumes, and energy metabolism. Methods: Eight isolated pig hearts were subjected to a protocol consisting of a 20-minute control perfusion, 120-minute SARC, and 20-minute reperfusion. The myocardial water content was monitored using near-infrared spectroscopy. Phosphorus-31 magnetic resonance (31P MR) spectroscopy was used to monitor the volumes of both intracellular and extracellular compartments and assess myocardial energy metabolism. Results: The near-infrared spectra showed that the 120-min SARC resulted in a 60 ± 12% increase in the myocardial water content. 31P MR spectra showed a 36 ± 4% increase in the intracellular compartment and a 54 ± 8% increase in the extracellular compartment during SARC relative to their initial volumes measured during control perfusion (100%). However, the myocardial energy metabolites (adenosine triphosphate [ATP] and phosphocreatine [PCr]) remained unchanged during the 120-minute SARC. Moreover, during reperfusion, the hearts showed an almost complete recovery in the left ventricular-developed pressure. Conclusions: A prolonged SARC resulted in water accumulation in both extracellular and intracellular compartments in the normal myocardium. Although its detrimental effect on tissue fluid homeostasis did not jeopardize the myocardial energy metabolism, a prolonged use of SARC should be avoided, particularly in the diseased hearts.

AB - Background and aim of the study: Simultaneous antegrade/retrograde cardioplegia (SARC) has been employed frequently during cardiac surgery to preserve the jeopardized myocardium. However, retrograde perfusion of SARC may interfere with myocardial drainage and disrupt myocardial fluid homeostasis, which may affect the myocardial energy metabolism and contractile function. The study was, therefore, designed to assess the effects of SARC on myocardial fluid homeostasis, cellular volumes, and energy metabolism. Methods: Eight isolated pig hearts were subjected to a protocol consisting of a 20-minute control perfusion, 120-minute SARC, and 20-minute reperfusion. The myocardial water content was monitored using near-infrared spectroscopy. Phosphorus-31 magnetic resonance (31P MR) spectroscopy was used to monitor the volumes of both intracellular and extracellular compartments and assess myocardial energy metabolism. Results: The near-infrared spectra showed that the 120-min SARC resulted in a 60 ± 12% increase in the myocardial water content. 31P MR spectra showed a 36 ± 4% increase in the intracellular compartment and a 54 ± 8% increase in the extracellular compartment during SARC relative to their initial volumes measured during control perfusion (100%). However, the myocardial energy metabolites (adenosine triphosphate [ATP] and phosphocreatine [PCr]) remained unchanged during the 120-minute SARC. Moreover, during reperfusion, the hearts showed an almost complete recovery in the left ventricular-developed pressure. Conclusions: A prolonged SARC resulted in water accumulation in both extracellular and intracellular compartments in the normal myocardium. Although its detrimental effect on tissue fluid homeostasis did not jeopardize the myocardial energy metabolism, a prolonged use of SARC should be avoided, particularly in the diseased hearts.

UR - http://www.scopus.com/inward/record.url?scp=50049128645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50049128645&partnerID=8YFLogxK

U2 - 10.1111/j.1540-8191.2008.00690.x

DO - 10.1111/j.1540-8191.2008.00690.x

M3 - Article

C2 - 18928482

AN - SCOPUS:50049128645

VL - 23

SP - 437

EP - 443

JO - Journal of Cardiac Surgery

JF - Journal of Cardiac Surgery

SN - 0886-0440

IS - 5

ER -