The effects of PPARC agonist and zinc on ovariectomized rats' vagina

Peter Takacs, Sindy Jaramillo, Yanping Zhang, Ram Datar, Anthony Williams, Joseph Olczyk, Keith A Candiotti, Carlos Medina

Research output: Contribution to journalArticle

Abstract

Objectives: This study aimed to measure the effects of peroxisome proliferator-activated receptor-C (PPARC) agonist GW501516 (GW) and zinc sulfate (ZS) on ovariectomized rats' vaginal histomorphology and collagen expression. Methods: Two weeks after ovariectomy, rats received daily treatment with vaginal suppositories containing placebo, ZS, GW, ZS with GW, or estradiol for 2 weeks. Macroscopic measurements were taken and the midsection of the vagina was used for histology. Immunofluorescence was performed with antibodies against collagen I, III, and anti-actin or collagen I and V and anti-actin. Gene expression analysis of 3 collagen genes was performed by qRT-PCR. Results: Macroscopic measurements revealed that the genital hiatus was narrower in the ZS and ZS with GW groups, and the vagina was significantly longer in the animals treated with GW, ZS with GW, and estradiol compared to the placebo group. Microscopic measurements of the vaginal layers showed that the lamina propria and the vaginal muscularis were significantly thicker in the ZS and ZS with GW group compared to the placebo. The ratio of vaginal Col1a1/Col3a1 mRNA expression was significantly up-regulated by ZS with GW compared to placebo, whereas the ratio of vaginal Col1a1/Col5a1 expression was significantly upregulated by ZS, GW, and ZS with GW. The ratio of vaginal collagen I/III protein expression was significantly up-regulated by ZS and ZS with GW, whereas the ratio of vaginal collagen I/V expression was significantly up-regulated by estradiol, ZS, and ZS with GW compared to control. Conclusions: Vaginal suppositories containing zinc and PPARC agonist significantly altered the vagina of ovariectomized rats.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalFemale Pelvic Medicine and Reconstructive Surgery
Volume19
Issue number3
DOIs
StatePublished - Jan 1 2013

Fingerprint

Zinc Sulfate
Peroxisome Proliferator-Activated Receptors
Vagina
Zinc
Collagen
Placebos
Suppositories
GW 501516
Actins
Estradiol
Ovariectomy

Keywords

  • Collagen
  • Peroxisome proliferator-activated receptor-C agonist
  • Vagina
  • Zinc

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Surgery
  • Urology

Cite this

The effects of PPARC agonist and zinc on ovariectomized rats' vagina. / Takacs, Peter; Jaramillo, Sindy; Zhang, Yanping; Datar, Ram; Williams, Anthony; Olczyk, Joseph; Candiotti, Keith A; Medina, Carlos.

In: Female Pelvic Medicine and Reconstructive Surgery, Vol. 19, No. 3, 01.01.2013, p. 126-131.

Research output: Contribution to journalArticle

Takacs, Peter ; Jaramillo, Sindy ; Zhang, Yanping ; Datar, Ram ; Williams, Anthony ; Olczyk, Joseph ; Candiotti, Keith A ; Medina, Carlos. / The effects of PPARC agonist and zinc on ovariectomized rats' vagina. In: Female Pelvic Medicine and Reconstructive Surgery. 2013 ; Vol. 19, No. 3. pp. 126-131.
@article{5624d741831c4bf3bd094fc1102aba55,
title = "The effects of PPARC agonist and zinc on ovariectomized rats' vagina",
abstract = "Objectives: This study aimed to measure the effects of peroxisome proliferator-activated receptor-C (PPARC) agonist GW501516 (GW) and zinc sulfate (ZS) on ovariectomized rats' vaginal histomorphology and collagen expression. Methods: Two weeks after ovariectomy, rats received daily treatment with vaginal suppositories containing placebo, ZS, GW, ZS with GW, or estradiol for 2 weeks. Macroscopic measurements were taken and the midsection of the vagina was used for histology. Immunofluorescence was performed with antibodies against collagen I, III, and anti-actin or collagen I and V and anti-actin. Gene expression analysis of 3 collagen genes was performed by qRT-PCR. Results: Macroscopic measurements revealed that the genital hiatus was narrower in the ZS and ZS with GW groups, and the vagina was significantly longer in the animals treated with GW, ZS with GW, and estradiol compared to the placebo group. Microscopic measurements of the vaginal layers showed that the lamina propria and the vaginal muscularis were significantly thicker in the ZS and ZS with GW group compared to the placebo. The ratio of vaginal Col1a1/Col3a1 mRNA expression was significantly up-regulated by ZS with GW compared to placebo, whereas the ratio of vaginal Col1a1/Col5a1 expression was significantly upregulated by ZS, GW, and ZS with GW. The ratio of vaginal collagen I/III protein expression was significantly up-regulated by ZS and ZS with GW, whereas the ratio of vaginal collagen I/V expression was significantly up-regulated by estradiol, ZS, and ZS with GW compared to control. Conclusions: Vaginal suppositories containing zinc and PPARC agonist significantly altered the vagina of ovariectomized rats.",
keywords = "Collagen, Peroxisome proliferator-activated receptor-C agonist, Vagina, Zinc",
author = "Peter Takacs and Sindy Jaramillo and Yanping Zhang and Ram Datar and Anthony Williams and Joseph Olczyk and Candiotti, {Keith A} and Carlos Medina",
year = "2013",
month = "1",
day = "1",
doi = "10.1097/SPV.0b013e31828746e9",
language = "English",
volume = "19",
pages = "126--131",
journal = "Female Pelvic Medicine and Reconstructive Surgery",
issn = "2151-8378",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - The effects of PPARC agonist and zinc on ovariectomized rats' vagina

AU - Takacs, Peter

AU - Jaramillo, Sindy

AU - Zhang, Yanping

AU - Datar, Ram

AU - Williams, Anthony

AU - Olczyk, Joseph

AU - Candiotti, Keith A

AU - Medina, Carlos

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Objectives: This study aimed to measure the effects of peroxisome proliferator-activated receptor-C (PPARC) agonist GW501516 (GW) and zinc sulfate (ZS) on ovariectomized rats' vaginal histomorphology and collagen expression. Methods: Two weeks after ovariectomy, rats received daily treatment with vaginal suppositories containing placebo, ZS, GW, ZS with GW, or estradiol for 2 weeks. Macroscopic measurements were taken and the midsection of the vagina was used for histology. Immunofluorescence was performed with antibodies against collagen I, III, and anti-actin or collagen I and V and anti-actin. Gene expression analysis of 3 collagen genes was performed by qRT-PCR. Results: Macroscopic measurements revealed that the genital hiatus was narrower in the ZS and ZS with GW groups, and the vagina was significantly longer in the animals treated with GW, ZS with GW, and estradiol compared to the placebo group. Microscopic measurements of the vaginal layers showed that the lamina propria and the vaginal muscularis were significantly thicker in the ZS and ZS with GW group compared to the placebo. The ratio of vaginal Col1a1/Col3a1 mRNA expression was significantly up-regulated by ZS with GW compared to placebo, whereas the ratio of vaginal Col1a1/Col5a1 expression was significantly upregulated by ZS, GW, and ZS with GW. The ratio of vaginal collagen I/III protein expression was significantly up-regulated by ZS and ZS with GW, whereas the ratio of vaginal collagen I/V expression was significantly up-regulated by estradiol, ZS, and ZS with GW compared to control. Conclusions: Vaginal suppositories containing zinc and PPARC agonist significantly altered the vagina of ovariectomized rats.

AB - Objectives: This study aimed to measure the effects of peroxisome proliferator-activated receptor-C (PPARC) agonist GW501516 (GW) and zinc sulfate (ZS) on ovariectomized rats' vaginal histomorphology and collagen expression. Methods: Two weeks after ovariectomy, rats received daily treatment with vaginal suppositories containing placebo, ZS, GW, ZS with GW, or estradiol for 2 weeks. Macroscopic measurements were taken and the midsection of the vagina was used for histology. Immunofluorescence was performed with antibodies against collagen I, III, and anti-actin or collagen I and V and anti-actin. Gene expression analysis of 3 collagen genes was performed by qRT-PCR. Results: Macroscopic measurements revealed that the genital hiatus was narrower in the ZS and ZS with GW groups, and the vagina was significantly longer in the animals treated with GW, ZS with GW, and estradiol compared to the placebo group. Microscopic measurements of the vaginal layers showed that the lamina propria and the vaginal muscularis were significantly thicker in the ZS and ZS with GW group compared to the placebo. The ratio of vaginal Col1a1/Col3a1 mRNA expression was significantly up-regulated by ZS with GW compared to placebo, whereas the ratio of vaginal Col1a1/Col5a1 expression was significantly upregulated by ZS, GW, and ZS with GW. The ratio of vaginal collagen I/III protein expression was significantly up-regulated by ZS and ZS with GW, whereas the ratio of vaginal collagen I/V expression was significantly up-regulated by estradiol, ZS, and ZS with GW compared to control. Conclusions: Vaginal suppositories containing zinc and PPARC agonist significantly altered the vagina of ovariectomized rats.

KW - Collagen

KW - Peroxisome proliferator-activated receptor-C agonist

KW - Vagina

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=84879748215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879748215&partnerID=8YFLogxK

U2 - 10.1097/SPV.0b013e31828746e9

DO - 10.1097/SPV.0b013e31828746e9

M3 - Article

C2 - 23611928

AN - SCOPUS:84879748215

VL - 19

SP - 126

EP - 131

JO - Female Pelvic Medicine and Reconstructive Surgery

JF - Female Pelvic Medicine and Reconstructive Surgery

SN - 2151-8378

IS - 3

ER -