The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity

P. Katz, A. M. Zaytoun, J. H. Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC), 11 normal adults received a single intravenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC using 51chromium (51Cr)-release and single cell cytotoxicity assays against Molt-4 and sensitized RL → target cells, respectively. Four hours after injection, both NK and ADCC activity were transiently increased in the 51Cr-release system (P < 0.05). At 4 hours, there was a twofold increase in the relative frequency of potentially cytotoxic target binding cells (P < 0.001) but the absolute number of these cells did not change (P < 0.1). However, the percentage lysis of bound targets at 4 hours was not altered (P > 0.1). These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) NK and ADCC activity did not differ in their responses to HC.

Original languageEnglish
Pages (from-to)72-78
Number of pages7
JournalArthritis and Rheumatism
Volume27
Issue number1
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Hydrocortisone
Lymphocytes
Antibodies
Natural Killer Cells
Sodium
Injections

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Katz, P., Zaytoun, A. M., & Lee, J. H. (1984). The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity. Arthritis and Rheumatism, 27(1), 72-78.

The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity. / Katz, P.; Zaytoun, A. M.; Lee, J. H.

In: Arthritis and Rheumatism, Vol. 27, No. 1, 01.01.1984, p. 72-78.

Research output: Contribution to journalArticle

Katz, P, Zaytoun, AM & Lee, JH 1984, 'The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity', Arthritis and Rheumatism, vol. 27, no. 1, pp. 72-78.
Katz, P. ; Zaytoun, A. M. ; Lee, J. H. / The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity. In: Arthritis and Rheumatism. 1984 ; Vol. 27, No. 1. pp. 72-78.
@article{d3e4fc770bd847ea9f8a1b141dd0fd35,
title = "The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity",
abstract = "To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC), 11 normal adults received a single intravenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC using 51chromium (51Cr)-release and single cell cytotoxicity assays against Molt-4 and sensitized RL → target cells, respectively. Four hours after injection, both NK and ADCC activity were transiently increased in the 51Cr-release system (P < 0.05). At 4 hours, there was a twofold increase in the relative frequency of potentially cytotoxic target binding cells (P < 0.001) but the absolute number of these cells did not change (P < 0.1). However, the percentage lysis of bound targets at 4 hours was not altered (P > 0.1). These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) NK and ADCC activity did not differ in their responses to HC.",
author = "P. Katz and Zaytoun, {A. M.} and Lee, {J. H.}",
year = "1984",
month = "1",
day = "1",
language = "English",
volume = "27",
pages = "72--78",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - The effects of in vivo hydrocortisone on lymphocyte-mediated cytotoxicity

AU - Katz, P.

AU - Zaytoun, A. M.

AU - Lee, J. H.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC), 11 normal adults received a single intravenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC using 51chromium (51Cr)-release and single cell cytotoxicity assays against Molt-4 and sensitized RL → target cells, respectively. Four hours after injection, both NK and ADCC activity were transiently increased in the 51Cr-release system (P < 0.05). At 4 hours, there was a twofold increase in the relative frequency of potentially cytotoxic target binding cells (P < 0.001) but the absolute number of these cells did not change (P < 0.1). However, the percentage lysis of bound targets at 4 hours was not altered (P > 0.1). These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) NK and ADCC activity did not differ in their responses to HC.

AB - To examine the effects of in vivo hydrocortisone sodium succinate (HC) on natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC), 11 normal adults received a single intravenous bolus of 400 mg hydrocortisone. Lymphocytes were tested for NK activity and ADCC using 51chromium (51Cr)-release and single cell cytotoxicity assays against Molt-4 and sensitized RL → target cells, respectively. Four hours after injection, both NK and ADCC activity were transiently increased in the 51Cr-release system (P < 0.05). At 4 hours, there was a twofold increase in the relative frequency of potentially cytotoxic target binding cells (P < 0.001) but the absolute number of these cells did not change (P < 0.1). However, the percentage lysis of bound targets at 4 hours was not altered (P > 0.1). These data suggest that: 1) lymphocytes participating in NK and ADCC reactions are refractory to the kinetic and functional effects of HC; 2) the increased lytic activity observed at 4 hours is due to a selective depletion of noncytotoxic cells from the circulation; and 3) NK and ADCC activity did not differ in their responses to HC.

UR - http://www.scopus.com/inward/record.url?scp=0021337904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021337904&partnerID=8YFLogxK

M3 - Article

C2 - 6691861

AN - SCOPUS:0021337904

VL - 27

SP - 72

EP - 78

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 1

ER -