The effects of glutamate receptor agonists on neurotensin release using in vivo microdialysis

James M. Radke, Michael J. Owens, Charles Nemeroff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the study, extracellular concentrations of neurotensin were measured from the striatum, nucleus accumbens and the medial prefrontal cortex in the awake, freely moving rat. Using a highly sensitive solid phase radioimmunoassay, basal concentrations of neurotensin were 2-5 pg/sample. In each region, glutamate receptor agonists, N-methyl-D-aspartate (NMDA) and kainic acid, increased neurotensin release 2-3-fold. Preincubation with the Na+ channel blocker tetrodotoxin abolished the glutamate receptor agonist-induced increases except in the striatum following kainic acid infusion. These findings indicate that activation of glutamate receptors may indirectly stimulate neurotensin release.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalEuropean Journal of Pharmacology
Volume411
Issue number1-2
DOIs
StatePublished - Jan 10 2001
Externally publishedYes

Fingerprint

Excitatory Amino Acid Agonists
Neurotensin
Microdialysis
Glutamate Receptors
Kainic Acid
Tetrodotoxin
Nucleus Accumbens
N-Methylaspartate
Prefrontal Cortex
Radioimmunoassay

Keywords

  • kainic acid
  • Medial prefontal cortex
  • NMDA (N-methyl-D-aspartate)
  • Nucleus accumbens
  • Striatum

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

The effects of glutamate receptor agonists on neurotensin release using in vivo microdialysis. / Radke, James M.; Owens, Michael J.; Nemeroff, Charles.

In: European Journal of Pharmacology, Vol. 411, No. 1-2, 10.01.2001, p. 129-134.

Research output: Contribution to journalArticle

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