The effects of elevated summer temperature and sublethal pollutants (ammonia, low pH) on protein turnover in the gill and liver of rainbow trout (Oncorhynchus mykiss) on a limited food ration

I. J. Morgan, L. M. D'Cruz, J. J. Dockray, T. K. Linton, C. M. Wood

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Protein synthesis, degradation and growth of the liver and gills were determined in juvenile rainbow trout (Oncorhynchus mykiss) fed a limited ration and exposed for 90 days to normal or elevated summer temperatures (+ 2°C above ambient) and either low pH (5.2) in softwater or 70 μM total ammonia in hardwater. The limited ration resulted in low rates of growth (< 0.80% per day) and protein synthesis in all fish. In softwater, whole-body growth was significantly inhibited by elevated temperature but stimulated by low pH, although tissue protein metabolism was generally unaffected by these treatments. There was no significant difference in final size between the groups of fish in hardwater, but liver protein synthesis and degradation were significantly lower at + 2°C, the reduction in synthesis being due to an inhibition of both the capacity for protein synthesis, C(s) and the RNA translational efficiency, k(RNA). Gill protein metabolism was unaffected by the experimental treatments in trout in hardwater. The authors conclude that a global warming scenario would be detrimental to protein synthesis and growth in freshwater fish under conditions of food limitation in summer, and when late summer temperatures approached the upper thermal limit of the species, regardless of food availability.

Original languageEnglish
Pages (from-to)43-53
Number of pages11
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume123
Issue number1
DOIs
StatePublished - May 1 1999

Fingerprint

Oncorhynchus mykiss
Ammonia
Liver
Food
Fishes
Temperature
Growth
Proteolysis
Proteins
Fish
RNA
Global Warming
Trout
Metabolism
Protein C
Fresh Water
Hot Temperature
Degradation
Global warming
Availability

Keywords

  • Ammonia
  • Oncorhynchus mykiss
  • pH
  • Protein synthesis
  • Rainbow trout
  • Ration
  • RNA
  • Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

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title = "The effects of elevated summer temperature and sublethal pollutants (ammonia, low pH) on protein turnover in the gill and liver of rainbow trout (Oncorhynchus mykiss) on a limited food ration",
abstract = "Protein synthesis, degradation and growth of the liver and gills were determined in juvenile rainbow trout (Oncorhynchus mykiss) fed a limited ration and exposed for 90 days to normal or elevated summer temperatures (+ 2°C above ambient) and either low pH (5.2) in softwater or 70 μM total ammonia in hardwater. The limited ration resulted in low rates of growth (< 0.80{\%} per day) and protein synthesis in all fish. In softwater, whole-body growth was significantly inhibited by elevated temperature but stimulated by low pH, although tissue protein metabolism was generally unaffected by these treatments. There was no significant difference in final size between the groups of fish in hardwater, but liver protein synthesis and degradation were significantly lower at + 2°C, the reduction in synthesis being due to an inhibition of both the capacity for protein synthesis, C(s) and the RNA translational efficiency, k(RNA). Gill protein metabolism was unaffected by the experimental treatments in trout in hardwater. The authors conclude that a global warming scenario would be detrimental to protein synthesis and growth in freshwater fish under conditions of food limitation in summer, and when late summer temperatures approached the upper thermal limit of the species, regardless of food availability.",
keywords = "Ammonia, Oncorhynchus mykiss, pH, Protein synthesis, Rainbow trout, Ration, RNA, Temperature",
author = "Morgan, {I. J.} and D'Cruz, {L. M.} and Dockray, {J. J.} and Linton, {T. K.} and Wood, {C. M.}",
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T1 - The effects of elevated summer temperature and sublethal pollutants (ammonia, low pH) on protein turnover in the gill and liver of rainbow trout (Oncorhynchus mykiss) on a limited food ration

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AU - D'Cruz, L. M.

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AU - Linton, T. K.

AU - Wood, C. M.

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N2 - Protein synthesis, degradation and growth of the liver and gills were determined in juvenile rainbow trout (Oncorhynchus mykiss) fed a limited ration and exposed for 90 days to normal or elevated summer temperatures (+ 2°C above ambient) and either low pH (5.2) in softwater or 70 μM total ammonia in hardwater. The limited ration resulted in low rates of growth (< 0.80% per day) and protein synthesis in all fish. In softwater, whole-body growth was significantly inhibited by elevated temperature but stimulated by low pH, although tissue protein metabolism was generally unaffected by these treatments. There was no significant difference in final size between the groups of fish in hardwater, but liver protein synthesis and degradation were significantly lower at + 2°C, the reduction in synthesis being due to an inhibition of both the capacity for protein synthesis, C(s) and the RNA translational efficiency, k(RNA). Gill protein metabolism was unaffected by the experimental treatments in trout in hardwater. The authors conclude that a global warming scenario would be detrimental to protein synthesis and growth in freshwater fish under conditions of food limitation in summer, and when late summer temperatures approached the upper thermal limit of the species, regardless of food availability.

AB - Protein synthesis, degradation and growth of the liver and gills were determined in juvenile rainbow trout (Oncorhynchus mykiss) fed a limited ration and exposed for 90 days to normal or elevated summer temperatures (+ 2°C above ambient) and either low pH (5.2) in softwater or 70 μM total ammonia in hardwater. The limited ration resulted in low rates of growth (< 0.80% per day) and protein synthesis in all fish. In softwater, whole-body growth was significantly inhibited by elevated temperature but stimulated by low pH, although tissue protein metabolism was generally unaffected by these treatments. There was no significant difference in final size between the groups of fish in hardwater, but liver protein synthesis and degradation were significantly lower at + 2°C, the reduction in synthesis being due to an inhibition of both the capacity for protein synthesis, C(s) and the RNA translational efficiency, k(RNA). Gill protein metabolism was unaffected by the experimental treatments in trout in hardwater. The authors conclude that a global warming scenario would be detrimental to protein synthesis and growth in freshwater fish under conditions of food limitation in summer, and when late summer temperatures approached the upper thermal limit of the species, regardless of food availability.

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