The effects of culture-maintained pancreatic islets on metabolic parameters, renal function, and glomerular lesions in the diabetic rat

H. P. Selawry, J. P. Pennell, V. Pardo, D. B. Alzamora, M. M. Millard

Research output: Contribution to journalArticle

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Abstract

This study was undertaken for two purposes: to determine whether cultured islets of adult donor rats could be used successfully to reverse the metabolic abnormalities of diabetes of long-term duration and to determine the effect of islet transplantation on renal function and glomerular lesions in diabetic rats. Four groups of rats were studied: (1) five age-matched normal control rats, (2) seven rats with chronic streptozotocin-induced diabetes, (3) six chronically diabetic rats transplant-improved for 3 months, and (4) five acutely diabetic rats transplant-improved for 12 months. Transplanted rats received 1500 culture-maintained adult pancreatic islets injected into the portal vein. Following successful islet transplantation, the metabolic parameters of diabetes, including hyperglycemia, glycosuria, and polyuria, returned to normal. Evaluation of renal function revealed that clearances of isotopic inulin and ρ{variant}-aminohippuric acid were not different among the groups. However, proteinuria was significantly greater in diabetic rats than ir both normal (p < 0.001) and transplant-improved rats (p < 0.01). There was striking glomerular immunofluorescence for IgG and C3 in diabetic rats and none in the other groups. Quantitative morphometric electron microscopy revealed that the glomerular mesangium was significantly greater than normal in diabetic rats and decreased in the transplant-improved rats. The data indicate that culture-maintained islets from adult donors, as previously reported for diabetes of short-term duration, have the capacity to reverse the diabetic process in chronically diabetic rats; and that although rats with chronic diabetes do not exhibit an alteration of glomerular filtration rate, they do develop marked proteinuria, glomerular immunofluorescence, and mesangial thickening, all of which improve following successful islet transplantation.

Original languageEnglish
Pages (from-to)261-269
Number of pages9
JournalMetabolism
Volume29
Issue number3
DOIs
StatePublished - Jan 1 1980

Fingerprint

Islets of Langerhans
Kidney
Islets of Langerhans Transplantation
Transplants
Proteinuria
Fluorescent Antibody Technique
Glomerular Mesangium
Tissue Donors
Glycosuria
p-Aminohippuric Acid
Polyuria
Inulin
Experimental Diabetes Mellitus
Portal Vein
Glomerular Filtration Rate
Hyperglycemia
Electron Microscopy
Immunoglobulin G

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The effects of culture-maintained pancreatic islets on metabolic parameters, renal function, and glomerular lesions in the diabetic rat. / Selawry, H. P.; Pennell, J. P.; Pardo, V.; Alzamora, D. B.; Millard, M. M.

In: Metabolism, Vol. 29, No. 3, 01.01.1980, p. 261-269.

Research output: Contribution to journalArticle

Selawry, H. P. ; Pennell, J. P. ; Pardo, V. ; Alzamora, D. B. ; Millard, M. M. / The effects of culture-maintained pancreatic islets on metabolic parameters, renal function, and glomerular lesions in the diabetic rat. In: Metabolism. 1980 ; Vol. 29, No. 3. pp. 261-269.
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