The effects of botulinum toxin A on muscle histology during distraction osteogenesis

Ronke Olabisi, Connie S. Chamberlain, Sarah Petr, Samuel Steiner, Daniel Consigny, Thomas Best, Ray Vanderby, Ed Schultz, Kenneth J. Noonan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Distraction osteogenesis is a highly successful method of bone formation, yet muscle fibrosis and contractures can result in significant morbidity. In the current study, we investigate the efficacy of botulinum toxin A in preventing fibrosis and potentially increasing muscle development in distracted muscles. Fifteen New Zealand White rabbits underwent tibial distraction at 1.5 mm/day until a 20% gain was achieved. Treatment groups were divided by drug (saline or botulinum toxin) and target muscle (gastrocnemius or tibialis anterior). Two additional control animals received no treatment. Bromeodeoxyuridine was delivered continuously throughout the 8-week experiment, and following muscle harvest. Tissues were stained for BrdU, Pax-7, vimentin, and haematoxylin and eosin staining. Mitotic activity increased in all distracted animals; however, in the animals receiving botulinum toxin A injections into the gastrocnemius, the antagonist tibialis anterior suffered up to 9% less fibrosis than distraction alone (p = 0.024). Use of botulinum A toxin did not appear to promote or improve neogenesis of muscle fibers, nor did it decrease fibrosis in the injected muscles. It appears from this study, and a previously published study on the effects of this toxin on muscle function, that botulinum A toxin maybe of some benefit in decreasing morbidity in the antagonist muscle but not the muscle injected with the toxin.

Original languageEnglish (US)
Pages (from-to)310-317
Number of pages8
JournalJournal of Orthopaedic Research
Volume27
Issue number3
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Fingerprint

Distraction Osteogenesis
Type A Botulinum Toxins
Histology
Muscles
Fibrosis
Morbidity
Botulinum Toxins
Muscle Development
Contracture
Vimentin
Bromodeoxyuridine
Hematoxylin
Eosine Yellowish-(YS)
Osteogenesis
Skeletal Muscle
Staining and Labeling
Rabbits
Injections

Keywords

  • Botulinum toxin
  • BrdU
  • Bromeodeoxyuridine
  • Distraction osteogenesis
  • Limb lengthening
  • Pax-7
  • Type A
  • Vimentin

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Olabisi, R., Chamberlain, C. S., Petr, S., Steiner, S., Consigny, D., Best, T., ... Noonan, K. J. (2009). The effects of botulinum toxin A on muscle histology during distraction osteogenesis. Journal of Orthopaedic Research, 27(3), 310-317. https://doi.org/10.1002/jor.20734

The effects of botulinum toxin A on muscle histology during distraction osteogenesis. / Olabisi, Ronke; Chamberlain, Connie S.; Petr, Sarah; Steiner, Samuel; Consigny, Daniel; Best, Thomas; Vanderby, Ray; Schultz, Ed; Noonan, Kenneth J.

In: Journal of Orthopaedic Research, Vol. 27, No. 3, 01.03.2009, p. 310-317.

Research output: Contribution to journalArticle

Olabisi, R, Chamberlain, CS, Petr, S, Steiner, S, Consigny, D, Best, T, Vanderby, R, Schultz, E & Noonan, KJ 2009, 'The effects of botulinum toxin A on muscle histology during distraction osteogenesis', Journal of Orthopaedic Research, vol. 27, no. 3, pp. 310-317. https://doi.org/10.1002/jor.20734
Olabisi, Ronke ; Chamberlain, Connie S. ; Petr, Sarah ; Steiner, Samuel ; Consigny, Daniel ; Best, Thomas ; Vanderby, Ray ; Schultz, Ed ; Noonan, Kenneth J. / The effects of botulinum toxin A on muscle histology during distraction osteogenesis. In: Journal of Orthopaedic Research. 2009 ; Vol. 27, No. 3. pp. 310-317.
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