The effect of (+)-tubocurarine on neuromuscular transmission during repetitive stimulation in the rat, mouse, and frog

Karl Magleby, B. S. Pallotta, D. A. Terrar

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The effect of tubocurarine on amplitudes of end-plate currents in response to trains of repetitive stimulation (50-150/sec) was investigated in voltage-clamped muscle fibres of the rat, mouse and frog. In rat and mouse muscle, the presence of tubocurarine led to a more rapid decline (rundown) in the amplitudes of successive end-plate currents during trains of impulses. In frog, tubocurarine caused an increase in apparent facilitation of end-plate current amplitudes during the first few impulses of repetitive stimulation; this increase was followed by a more rapid rundown of end-plate current amplitude. These effects of tubocurarine appear not to be an artifact resulting from inadequate control of membrane potential in voltage-clamped fibres. The more rapid rundown during trains of end-plate currents in the presence of tubocurarine showed little variation with membrane potential indicating that voltage-sensitive channel blockade by tubocurarine was not a major factor contributing to the rundown. The effect of tubocurarine on the apparent facilitation and rundown of end-plate current amplitudes was typically decreased by reducing the frequency of stimulation. These results suggest that tubocurarine effects transmitter release at neuromuscular junctions during repetitive stimulation.

Original languageEnglish
Pages (from-to)97-113
Number of pages17
JournalJournal of Physiology
VolumeVol.312
StatePublished - Jan 1 1981
Externally publishedYes

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Tubocurarine
Anura
Membrane Potentials
Muscles
Neuromuscular Junction
Artifacts

ASJC Scopus subject areas

  • Physiology

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The effect of (+)-tubocurarine on neuromuscular transmission during repetitive stimulation in the rat, mouse, and frog. / Magleby, Karl; Pallotta, B. S.; Terrar, D. A.

In: Journal of Physiology, Vol. Vol.312, 01.01.1981, p. 97-113.

Research output: Contribution to journalArticle

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