The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry

Octavio V. Martinez, H. G. Gratzner, Theodore Malinin, M. Ingram

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Citations (Scopus)

Abstract

The effects of three beta-lactam antibodies on E. coli were studied by means of flow cytometry. Since these agents block bacterial cell wall synthesis in such manner as to prevent septal formation without appreciably affecting nucleic acid synthesis, the resulting cell elongation caused by these agents can be assessed by nucleic acid fluorescent staining. It was shown by this technique that the somatic effects of cefazolin, cefamandole and moxalactam were related both to the antibiotic concentration and time of exposure to the drugs and were observable within 30 minutes of the initial exposure of the cultures to these agents. These results demonstrate that fluorescent cytometry can provide accurate assessment of the effects of compounds that inhibit cell wall formation. This technology could be a useful tool for comparing antibiotic somatic effects on bacteria and for rapidly and reliably determining their sensitivity and resistance to these agents.

Original languageEnglish
Title of host publicationCytometry
Pages129-133
Number of pages5
Volume3
Edition2
StatePublished - Dec 1 1982

Fingerprint

Lactams
Cell Wall
Nucleic Acids
Flow Cytometry
Cefamandole
Moxalactam
Escherichia coli
Anti-Bacterial Agents
Cefazolin
beta-Lactams
Staining and Labeling
Technology
Bacteria
Antibodies
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Martinez, O. V., Gratzner, H. G., Malinin, T., & Ingram, M. (1982). The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry. In Cytometry (2 ed., Vol. 3, pp. 129-133)

The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry. / Martinez, Octavio V.; Gratzner, H. G.; Malinin, Theodore; Ingram, M.

Cytometry. Vol. 3 2. ed. 1982. p. 129-133.

Research output: Chapter in Book/Report/Conference proceedingChapter

Martinez, OV, Gratzner, HG, Malinin, T & Ingram, M 1982, The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry. in Cytometry. 2 edn, vol. 3, pp. 129-133.
Martinez OV, Gratzner HG, Malinin T, Ingram M. The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry. In Cytometry. 2 ed. Vol. 3. 1982. p. 129-133
Martinez, Octavio V. ; Gratzner, H. G. ; Malinin, Theodore ; Ingram, M. / The effect of some β-lactam antibiotics on Escherichia coli studied by flow cytometry. Cytometry. Vol. 3 2. ed. 1982. pp. 129-133
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