The effect of short-term hypoxic exposure on metabolic gene expression

Meredith V. Everett, Corina E. Antal, Douglas L Crawford

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The long-term effect of hypoxia is to decrease both the production and use of ATP and thus decrease the reliance on mitochondrial oxidative energy production. Yet, recent studies include more immediate affects of hypoxia on gene expression and these data suggest the maintenance of mitochondrial function. To better understand the short-term physiological response to hypoxia, we quantified metabolic mRNA expression in the heart ventricles and livers of the teleost fish Fundulus grandis exposed to partial oxygen pressure of 2.8kPa (∼13.5% air saturation).Twenty-eight individuals from a single population were exposed to hypoxia for 0, 4, 8, 12, 24, 48, and 96hr. Liver and cardiac tissues were sampled from the same individuals at 0-48hr. At 96hr, only cardiac tissue was assayed. Gene expression was significantly different (ANOVA, P<0.05) for 17 of 226 metabolic genes (7.5%) in cardiac tissue and for 20 of 256 (7.8%) metabolic genes in hepatic tissue. For the two tissues examined in this study, the maximum response occurred at different times. For cardiac tissue, using Dunnett's post hoc test, most of these significant differences occurred at 96hr of exposure. For liver, all but one significant difference occurred at 4hr. Surprisingly, too many (relative to random expectations) of the genes with significant increase in mRNA are involved in the oxidative phosphorylation pathway: 44% of the significant genes at 96hr in the heart and 33% of the significant genes at 4hr in the liver are involved in the oxidative phosphorylation pathway. These data indicate that there are tissue-specific differences in the timing of the response to hypoxia, yet both cardiac and hepatic tissues have increases in mRNA that code for enzyme in the oxidative phosphorylation pathway. If these changes in mRNA produce a similar change in protein, then these data suggest that the initial response to hypoxia involves an increase in the oxidative pathway potentially as a mechanism to maintain ATP production.

Original languageEnglish
Pages (from-to)9-23
Number of pages15
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume317 A
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

gene expression
hypoxia
Gene Expression
liver
oxidative phosphorylation
Liver
Oxidative Phosphorylation
gene
Messenger RNA
Genes
genes
Fundulus grandis
Adenosine Triphosphate
Fundulidae
heart ventricle
tissues
tissue
effect
exposure
Partial Pressure

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Physiology
  • Molecular Biology

Cite this

The effect of short-term hypoxic exposure on metabolic gene expression. / Everett, Meredith V.; Antal, Corina E.; Crawford, Douglas L.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 317 A, No. 1, 01.01.2012, p. 9-23.

Research output: Contribution to journalArticle

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