The effect of octreotide on hepatic ischemia-reperfusion injury in a rabbit model

J. Yang, H. Sun, P. Takacs, Y. Zhang, J. Liu, Y. Chang, K. A. Candiotti

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Background Hepatic ischemic-reperfusion injury (HIRI) is a major cause of morbidity and mortality following liver surgery. Octreotide (Oct) has been reported to improve hepatocellular energy metabolism in a rat HIRI model. This study was designed to evaluate whether Oct could protect the liver of rabbits against ischemic-reperfusion (I/R) injury. Methods Twenty-four adult New Zealand rabbits were randomly divided into a sham operated group (Control), an ischemia/reperfusion group (I/R), and an ischemia/reperfusion + Oct pretreatment group (I/R + Oct). The hemodynamic (mean arterial pressure [MAP] and heart rate [HR]) changes, liver enzymes (alanine aminotransferase [ALT], aspartate aminotransferase [AST], and lactate dehydrogenase [LDH]) release, inflammatory cytokines (tumor necrosis factor [TNF]α and interleukin [IL]-1β) levels, and endotoxin (ETX) levels were measured during I/R. Results Compared with the Control group, the MAP decreased and HR increased in I/R and I/R + Oct groups at ischemia 15 minutes (P <.05) but were less in the I/R + Oct group relative to the I/R group (P <.05). ALT, AST, LDH, IL-1β, and ETX levels were increased in the I/R and I/R + Oct groups at ischemia 30 minutes (P <.05), however, the increase was lower in the I/R + Oct group relative to the I/R group (P <.05). Bcl-2 expression in the I/R + Oct group was higher compared with other groups (P <.05) and Bax expression in the I/R group was reduced compared with other groups (P <.05). Hepatocellular damage in the I/R + Oct group appeared to be less than in the I/R group by microscopy. Conclusions Oct pretreatment attenuated hemodynamic changes and decreased liver enzyme changes induced by HIRI in a rabbit model. The protection mechanisms of Oct may be related to reduced ETX levels, down-regulation of the inflammatory cytokines TNFα and IL-1β, and inhibition of hepatocellular apoptosis, as well as the modulation of the mitochondrion-mediated Bcl-2/Bax apoptosis pathway. Based on our study it appears that Oct may be useful in decreasing liver injury after liver surgery and/or transplantation and may serve as a promising agent against HIRI.

Original languageEnglish (US)
Pages (from-to)2433-2438
Number of pages6
JournalTransplantation proceedings
Volume45
Issue number6
DOIs
StatePublished - Jul 1 2013

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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