The effect of nicotine on the mechanical properties of mesenchymal stem cells

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. Methods: MSCs were synchronized to the G0 stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 μM, 0.5 μM, and 1 μM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. Results: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 μM and 1.0 μM concentrations only. Conclusions: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalCell Health and Cytoskeleton
Volume4
DOIs
StatePublished - Dec 1 2012

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Nicotine
Cytoplasm
Cell Nucleus
Mechanical properties
Cells
Atomic Force Microscopy
Elastic Modulus
Elasticity
Atomic force microscopy
Cell Cycle
Elastic moduli
Serum

Keywords

  • Atomic force microscopy
  • Elasticity
  • Mesenchymal stem cells
  • Nicotine

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology
  • Histology
  • Biochemistry

Cite this

The effect of nicotine on the mechanical properties of mesenchymal stem cells. / Ruiz, Juan P.; Pelaez, Daniel; Dias, Janice; Ziebarth, Noel Marysa; Cheung, Herman S.

In: Cell Health and Cytoskeleton, Vol. 4, 01.12.2012, p. 29-35.

Research output: Contribution to journalArticle

@article{b01ff07e235d43e2bc45d9e4ed9d8e28,
title = "The effect of nicotine on the mechanical properties of mesenchymal stem cells",
abstract = "Purpose: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. Methods: MSCs were synchronized to the G0 stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 μM, 0.5 μM, and 1 μM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. Results: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 μM and 1.0 μM concentrations only. Conclusions: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.",
keywords = "Atomic force microscopy, Elasticity, Mesenchymal stem cells, Nicotine",
author = "Ruiz, {Juan P.} and Daniel Pelaez and Janice Dias and Ziebarth, {Noel Marysa} and Cheung, {Herman S}",
year = "2012",
month = "12",
day = "1",
doi = "10.2147/CHC.S24381",
language = "English",
volume = "4",
pages = "29--35",
journal = "Cell Health and Cytoskeleton",
issn = "1179-1330",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - The effect of nicotine on the mechanical properties of mesenchymal stem cells

AU - Ruiz, Juan P.

AU - Pelaez, Daniel

AU - Dias, Janice

AU - Ziebarth, Noel Marysa

AU - Cheung, Herman S

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Purpose: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. Methods: MSCs were synchronized to the G0 stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 μM, 0.5 μM, and 1 μM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. Results: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 μM and 1.0 μM concentrations only. Conclusions: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.

AB - Purpose: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. Methods: MSCs were synchronized to the G0 stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 μM, 0.5 μM, and 1 μM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. Results: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 μM and 1.0 μM concentrations only. Conclusions: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.

KW - Atomic force microscopy

KW - Elasticity

KW - Mesenchymal stem cells

KW - Nicotine

UR - http://www.scopus.com/inward/record.url?scp=84874091044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874091044&partnerID=8YFLogxK

U2 - 10.2147/CHC.S24381

DO - 10.2147/CHC.S24381

M3 - Article

AN - SCOPUS:84874091044

VL - 4

SP - 29

EP - 35

JO - Cell Health and Cytoskeleton

JF - Cell Health and Cytoskeleton

SN - 1179-1330

ER -