The effect of methylprednisolone on caspase-3 activation after rat spinal cord transection

X. Li, Martin Oudega, H. A. Dancausse, Allan D Levi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: Caspase-3 is known as a crucial effector for apoptotic cell death. Apoptosis has recently been recognized as an important cell death mechanism after spinal cord injury (SCI). This study attempts to define the effect of methylprednisolone (MP) on the activation of caspase-3 in the lesioned area following SCI. Methods: Forty-eight rats with a complete transection of the thoracic spinal cord received a placebo or MP (30 mg/kg, iv.) at 5 min, 2 and 4 h post-injury and were then sacrificed at 12, 24 h, 3 or 7 days thereafter. Results: Caspase-3 positive cells in the lesioned area were immunocytochemically observed in both cord stumps and decreased in number with increasing distance from the lesion site. More caspase-3 positive cells were present in the MP-treated group than the control group at all time points, but the differences were not statistically significant. Conclusion: These results suggest that the MP-induced decrease of tissue loss following SCI may not involve a reduction of apoptotic cell death.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalRestorative Neurology and Neuroscience
Volume17
Issue number4
StatePublished - Dec 1 2000

Fingerprint

Methylprednisolone
Spinal Cord Injuries
Caspase 3
Cell Death
Thorax
Placebos
Apoptosis
Control Groups
Wounds and Injuries

Keywords

  • Apoptosis
  • Caspase-3
  • Immunocytochemistry
  • Methylprednisolone
  • Secondary damage
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology

Cite this

The effect of methylprednisolone on caspase-3 activation after rat spinal cord transection. / Li, X.; Oudega, Martin; Dancausse, H. A.; Levi, Allan D.

In: Restorative Neurology and Neuroscience, Vol. 17, No. 4, 01.12.2000, p. 203-209.

Research output: Contribution to journalArticle

@article{ac8b63bbf12d42d881cecc7b04599251,
title = "The effect of methylprednisolone on caspase-3 activation after rat spinal cord transection",
abstract = "Purpose: Caspase-3 is known as a crucial effector for apoptotic cell death. Apoptosis has recently been recognized as an important cell death mechanism after spinal cord injury (SCI). This study attempts to define the effect of methylprednisolone (MP) on the activation of caspase-3 in the lesioned area following SCI. Methods: Forty-eight rats with a complete transection of the thoracic spinal cord received a placebo or MP (30 mg/kg, iv.) at 5 min, 2 and 4 h post-injury and were then sacrificed at 12, 24 h, 3 or 7 days thereafter. Results: Caspase-3 positive cells in the lesioned area were immunocytochemically observed in both cord stumps and decreased in number with increasing distance from the lesion site. More caspase-3 positive cells were present in the MP-treated group than the control group at all time points, but the differences were not statistically significant. Conclusion: These results suggest that the MP-induced decrease of tissue loss following SCI may not involve a reduction of apoptotic cell death.",
keywords = "Apoptosis, Caspase-3, Immunocytochemistry, Methylprednisolone, Secondary damage, Spinal cord injury",
author = "X. Li and Martin Oudega and Dancausse, {H. A.} and Levi, {Allan D}",
year = "2000",
month = "12",
day = "1",
language = "English",
volume = "17",
pages = "203--209",
journal = "Restorative Neurology and Neuroscience",
issn = "0922-6028",
publisher = "IOS Press",
number = "4",

}

TY - JOUR

T1 - The effect of methylprednisolone on caspase-3 activation after rat spinal cord transection

AU - Li, X.

AU - Oudega, Martin

AU - Dancausse, H. A.

AU - Levi, Allan D

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Purpose: Caspase-3 is known as a crucial effector for apoptotic cell death. Apoptosis has recently been recognized as an important cell death mechanism after spinal cord injury (SCI). This study attempts to define the effect of methylprednisolone (MP) on the activation of caspase-3 in the lesioned area following SCI. Methods: Forty-eight rats with a complete transection of the thoracic spinal cord received a placebo or MP (30 mg/kg, iv.) at 5 min, 2 and 4 h post-injury and were then sacrificed at 12, 24 h, 3 or 7 days thereafter. Results: Caspase-3 positive cells in the lesioned area were immunocytochemically observed in both cord stumps and decreased in number with increasing distance from the lesion site. More caspase-3 positive cells were present in the MP-treated group than the control group at all time points, but the differences were not statistically significant. Conclusion: These results suggest that the MP-induced decrease of tissue loss following SCI may not involve a reduction of apoptotic cell death.

AB - Purpose: Caspase-3 is known as a crucial effector for apoptotic cell death. Apoptosis has recently been recognized as an important cell death mechanism after spinal cord injury (SCI). This study attempts to define the effect of methylprednisolone (MP) on the activation of caspase-3 in the lesioned area following SCI. Methods: Forty-eight rats with a complete transection of the thoracic spinal cord received a placebo or MP (30 mg/kg, iv.) at 5 min, 2 and 4 h post-injury and were then sacrificed at 12, 24 h, 3 or 7 days thereafter. Results: Caspase-3 positive cells in the lesioned area were immunocytochemically observed in both cord stumps and decreased in number with increasing distance from the lesion site. More caspase-3 positive cells were present in the MP-treated group than the control group at all time points, but the differences were not statistically significant. Conclusion: These results suggest that the MP-induced decrease of tissue loss following SCI may not involve a reduction of apoptotic cell death.

KW - Apoptosis

KW - Caspase-3

KW - Immunocytochemistry

KW - Methylprednisolone

KW - Secondary damage

KW - Spinal cord injury

UR - http://www.scopus.com/inward/record.url?scp=0034437887&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034437887&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034437887

VL - 17

SP - 203

EP - 209

JO - Restorative Neurology and Neuroscience

JF - Restorative Neurology and Neuroscience

SN - 0922-6028

IS - 4

ER -