The effect of lipid on chymotrypsin assay in stools

Arvey I. Rogers, Paul S. Bachorik, Derk A. Vloedman, Majoin H. Kalser

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Abstract

The sodium salt of oleic acid has been demonstrated to interfere with the measurement of the esterolytic activity of chymotrypsin on a specific substrate, ATEE, utilizing automatic titration. The inhibitory effect of sodium oleate has been attributed in part to its ability to act as a buffering agent in an assay system, since nonlipid buffers (tris)-in increasing concentration-exerted a similar effect. Fecal samples with high lipid content decreased the recovery of added chymotrypsin. Successive dilutions of fecal samples resulted in an increase in assayable native chymotrypsin activity. Stool samples varying in lipid content and buffer capacity may help explain low fecal chymotrypsin recoveries observed in nonpancreatogenous steatorrhea.

Original languageEnglish (US)
Pages (from-to)664-673
Number of pages10
JournalThe American Journal of Digestive Diseases
Volume12
Issue number7
DOIs
StatePublished - Jul 1 1967

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ASJC Scopus subject areas

  • Gastroenterology

Cite this

Rogers, A. I., Bachorik, P. S., Vloedman, D. A., & Kalser, M. H. (1967). The effect of lipid on chymotrypsin assay in stools. The American Journal of Digestive Diseases, 12(7), 664-673. https://doi.org/10.1007/BF02238274