The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo

In Kap Ko, Bu Kyu Lee, Sang Jin Lee, Karl Erik Andersson, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Timely innervation of muscle tissue is critical in the recovery of function, and this time-sensitive process relies heavily on the host tissue microenvironment after implantation. However, restoration of muscle tissue mass and function has been a challenge. We investigated whether pre-forming acetylcholine receptor (AChR) clusters on engineered muscle fibers using an AChR cluster-inducing factor (agrin) prior to implantation would facilitate established contacts between implanted muscle tissues and nerves and result in rapid innervation of engineered muscle in vivo. We showed that agrin treatment significantly increased the formation of AChR clusters on culture differentiated myotubes (C2C12), enhanced contacts with nerves in vitro and in vivo, and increased angiogenesis. Pre-fabrication of AChR clusters on engineered skeletal muscle using a released neurotrophic factor can accelerate innervations following implantation in vivo. This technique has considerable potential for enhancing muscle tissue function.

Original languageEnglish
Pages (from-to)3246-3255
Number of pages10
JournalBiomaterials
Volume34
Issue number13
DOIs
StatePublished - Apr 1 2013
Externally publishedYes

Fingerprint

Cholinergic Receptors
Muscle
Muscles
Fibers
Agrin
Tissue
Skeletal Muscle Fibers
Recovery of Function
Nerve Growth Factors
In Vitro Techniques
Skeletal Muscle
Restoration
Fabrication
Recovery

Keywords

  • Angiogenesis
  • In vivo test
  • Muscle
  • Neural network

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo. / Ko, In Kap; Lee, Bu Kyu; Lee, Sang Jin; Andersson, Karl Erik; Atala, Anthony; Yoo, James J.

In: Biomaterials, Vol. 34, No. 13, 01.04.2013, p. 3246-3255.

Research output: Contribution to journalArticle

Ko, In Kap ; Lee, Bu Kyu ; Lee, Sang Jin ; Andersson, Karl Erik ; Atala, Anthony ; Yoo, James J. / The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo. In: Biomaterials. 2013 ; Vol. 34, No. 13. pp. 3246-3255.
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