Abstract
Timely innervation of muscle tissue is critical in the recovery of function, and this time-sensitive process relies heavily on the host tissue microenvironment after implantation. However, restoration of muscle tissue mass and function has been a challenge. We investigated whether pre-forming acetylcholine receptor (AChR) clusters on engineered muscle fibers using an AChR cluster-inducing factor (agrin) prior to implantation would facilitate established contacts between implanted muscle tissues and nerves and result in rapid innervation of engineered muscle in vivo. We showed that agrin treatment significantly increased the formation of AChR clusters on culture differentiated myotubes (C2C12), enhanced contacts with nerves in vitro and in vivo, and increased angiogenesis. Pre-fabrication of AChR clusters on engineered skeletal muscle using a released neurotrophic factor can accelerate innervations following implantation in vivo. This technique has considerable potential for enhancing muscle tissue function.
| Original language | English |
|---|---|
| Pages (from-to) | 3246-3255 |
| Number of pages | 10 |
| Journal | Biomaterials |
| Volume | 34 |
| Issue number | 13 |
| DOIs | |
| State | Published - Apr 1 2013 |
| Externally published | Yes |
Fingerprint
Keywords
- Angiogenesis
- In vivo test
- Muscle
- Neural network
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo. / Ko, In Kap; Lee, Bu Kyu; Lee, Sang Jin; Andersson, Karl Erik; Atala, Anthony; Yoo, James J.
In: Biomaterials, Vol. 34, No. 13, 01.04.2013, p. 3246-3255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of in vitro formation of acetylcholine receptor (AChR) clusters in engineered muscle fibers on subsequent innervation of constructs in vivo
AU - Ko, In Kap
AU - Lee, Bu Kyu
AU - Lee, Sang Jin
AU - Andersson, Karl Erik
AU - Atala, Anthony
AU - Yoo, James J.
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Timely innervation of muscle tissue is critical in the recovery of function, and this time-sensitive process relies heavily on the host tissue microenvironment after implantation. However, restoration of muscle tissue mass and function has been a challenge. We investigated whether pre-forming acetylcholine receptor (AChR) clusters on engineered muscle fibers using an AChR cluster-inducing factor (agrin) prior to implantation would facilitate established contacts between implanted muscle tissues and nerves and result in rapid innervation of engineered muscle in vivo. We showed that agrin treatment significantly increased the formation of AChR clusters on culture differentiated myotubes (C2C12), enhanced contacts with nerves in vitro and in vivo, and increased angiogenesis. Pre-fabrication of AChR clusters on engineered skeletal muscle using a released neurotrophic factor can accelerate innervations following implantation in vivo. This technique has considerable potential for enhancing muscle tissue function.
AB - Timely innervation of muscle tissue is critical in the recovery of function, and this time-sensitive process relies heavily on the host tissue microenvironment after implantation. However, restoration of muscle tissue mass and function has been a challenge. We investigated whether pre-forming acetylcholine receptor (AChR) clusters on engineered muscle fibers using an AChR cluster-inducing factor (agrin) prior to implantation would facilitate established contacts between implanted muscle tissues and nerves and result in rapid innervation of engineered muscle in vivo. We showed that agrin treatment significantly increased the formation of AChR clusters on culture differentiated myotubes (C2C12), enhanced contacts with nerves in vitro and in vivo, and increased angiogenesis. Pre-fabrication of AChR clusters on engineered skeletal muscle using a released neurotrophic factor can accelerate innervations following implantation in vivo. This technique has considerable potential for enhancing muscle tissue function.
KW - Angiogenesis
KW - In vivo test
KW - Muscle
KW - Neural network
UR - http://www.scopus.com/inward/record.url?scp=84874278618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874278618&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2013.01.029
DO - 10.1016/j.biomaterials.2013.01.029
M3 - Article
C2 - 23391495
AN - SCOPUS:84874278618
VL - 34
SP - 3246
EP - 3255
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 13
ER -