The effect of high K+ depolarization and verapamil on45Ca2+ fluxes in the canine myocardium

N. J. Lodge, A. L. Bassett, H. Gelband

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effects of high K+ depolarization and verapamil on Ca2+ uptake and the total intracellular Ca2+ content of canine ventricular muscle strips (~0.5 mm thick) were investigated. High K+ (96 mM) increased Ca2+ uptake above control and maintained this enhanced uptake throughout a 90 sec measuring period. Verapamil (5 x 10-6 M) significantly (p < 0.05) inhibited this high K+ stimulated uptake. However, verapamil (5 x 10-6 M) also had a direct effect on Ca2+ fluxes, causing a significant increase in both Ca2+ uptake (p < 0.001) and total intracellular Ca2+ content (p < 0.001) in the resting tissue. Therefore, verapamil's apparent inhibition of high K+ stimulated Ca2+ uptake may have resulted from some mechanism other than Ca2+ channel blockade.

Original languageEnglish (US)
Pages (from-to)222-224
Number of pages3
JournalPflügers Archiv: European Journal of Physiology
Volume403
Issue number2
DOIs
StatePublished - Feb 1985

Keywords

  • Calcium
  • Canine ventricular muscle
  • Potassium depolarization
  • Verapamil

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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