The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype

Catharina Adelöw, Tatiana Segura, Jeffrey A. Hubbell, Peter Frey

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The formation of scar tissue due to dedifferentiation of smooth muscle cells (SMCs) is one of the major issues faced when engineering bladder tissue. Furthermore, cell sources for regenerating the SMC layer are also limiting. Here we explore if human mesenchymal stem cells (MCSs), cultured in enzymatically degradable poly(ethylene glycol) (PEG) hydrogel scaffolds can be differentiated into SMC-like cells. We explored the degree to which a less synthetic SMC phenotype can be achieved when primary human SMCs are cultured within these scaffolds, It was observed that when both MSCs and SMCs are cultured in the PEG hydrogel scaffolds, but not on traditional tissue culture plastic, they up-regulate markers associated with the less synthetic SMC phenotype, decreased expression of α5 integrin and THY-1, and increased expression of α-smooth muscle actin (αSMA) and myosin. Furthermore, we show that MSCs and SMCs cultured in the PEG hydrogels are able to proliferate and express matrix metalloproteinases for up to 21 d in culture, the duration of the study. This study addresses the importance of the cellular microenvironment on cell fate, and proposes synthetic instructive biomaterials as a means to direct cell differentiation and circumvent scar tissue formation during bladder reconstruction.

Original languageEnglish
Pages (from-to)314-326
Number of pages13
JournalBiomaterials
Volume29
Issue number3
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Hydrogels
Ethylene Glycol
Polyethylene glycols
Smooth Muscle Myocytes
Muscle
Cells
Phenotype
Hydrogel
Scaffolds
Cicatrix
Urinary Bladder
Tissue
Smooth Muscle Myosins
Cellular Microenvironment
Tissue culture
Biocompatible Materials
Tissue Engineering
Scaffolds (biology)
Stem cells
Matrix Metalloproteinases

Keywords

  • Bladder
  • ECM
  • Hydrogel
  • Mesenchymal stem cell
  • PEG
  • Smooth muscle cell

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype. / Adelöw, Catharina; Segura, Tatiana; Hubbell, Jeffrey A.; Frey, Peter.

In: Biomaterials, Vol. 29, No. 3, 01.01.2008, p. 314-326.

Research output: Contribution to journalArticle

Adelöw, Catharina ; Segura, Tatiana ; Hubbell, Jeffrey A. ; Frey, Peter. / The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype. In: Biomaterials. 2008 ; Vol. 29, No. 3. pp. 314-326.
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