The effect of enoxaparin on bleomycin-induced lung injury in mice

U. Laxer, I. S. Lossos, S. Gillis, R. Or, T. G. Christensen, R. H. Goldstein, Raphael Breuer

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have evaluated the effect of enoxaparin, a potent antithrombotic drug, on bleomycin (Bleo)-induced pulmonary inflammation in mice. Pulmonary injury was induced by a single intratracheal (IT) instillation of Bleo. Four groups of female C57BL/6 mice, each received one of four treatments: (1) IT Bleo and daily intraperitoneal (IP) injections of enoxaparin (EN) starting one day before IT instillation of Bleo (Bleo-EN); (2) IT Bleo and IP injections of saline (Bleo-Sal); (3) IT saline and IP enoxaparin (Sal-EN); (4) IT saline and IP saline (Sal-Sal). Animals were sacrificed 14 days after IT treatment. Lung injury was evaluated by analysis of bronchoalveolar lavage fluid and histologically by an overall semiquantitative index of lung injury and a quantitative image analysis assessing alveolar wall area fraction and fibrosis fraction. Treatment of mice with enoxaparin did not ameliorate Bleoinduced lung injury. Our study does not establish a critical role of procoagulant activity in the evolution of Bleo-induced lung injury and does not support the use of antithrombotic therapy for the prevention of pulmonary fibrosis.

Original languageEnglish (US)
Pages (from-to)531-541
Number of pages11
JournalExperimental Lung Research
Volume25
Issue number6
DOIs
StatePublished - Oct 5 1999
Externally publishedYes

Keywords

  • Bleomycin
  • Enoxaparin
  • Interstitial lung disease
  • Low-molecular-weight heparin
  • Mice

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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  • Cite this

    Laxer, U., Lossos, I. S., Gillis, S., Or, R., Christensen, T. G., Goldstein, R. H., & Breuer, R. (1999). The effect of enoxaparin on bleomycin-induced lung injury in mice. Experimental Lung Research, 25(6), 531-541. https://doi.org/10.1080/019021499270114