The effect of corticosteroids on the disposal of long-acting β2-agonists by airway smooth muscle cells

Gabor Horvath, Eliana S. Mendes, Nathalie Schmid, Andreas Schmid, Gregory E. Conner, Matthias Salathe, Adam Wanner

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Background: Organic cation transporters (OCTs) have an important role in tissue distribution and elimination of cationic drugs. Carrier-mediated disposal of cationic bronchodilators in the airway tissue, however, is incompletely understood. Objectives: We sought to assess the uptake of long-acting β2-agonist bronchodilators by bronchial and vascular smooth muscle cells. Methods: Human airway cells and tissues obtained from organ donors were evaluated for cationic drug transporter expression by means of quantitative RT-PCR and immunofluorescence. For in vitro functional studies, tritiated formoterol and tritiated salmeterol uptake by bronchial and vascular smooth muscle cells was measured. Results: Quantitative RT-PCR analysis revealed high mRNA levels for the corticosteroid-sensitive OCT3 in bronchial and vascular smooth muscle cells. Immunofluorescence staining of airway sections confirmed OCT3 expression in these cells. In bronchial smooth muscle cells, uptake of the cationic formoterol was inhibited with OCT inhibitors. Corticosteroids also inhibited formoterol uptake through a rapid (within 15 minutes) nongenomic action, with the following rank order for inhibitory potency: corticosterone > budesonide > fluticasone. The corticosteroid-induced inhibition was significantly higher in vascular than bronchial smooth muscle cells. In comparison with formoterol, uptake of the noncharged lipophilic salmeterol was approximately 10-fold higher and insensitive to all OCT inhibitors and corticosteroids. Conclusions: Our findings suggest that corticosteroids, through OCT3 inhibition, rapidly interfere with the disposal of cationic drugs by smooth muscle cells in the airway. Clinical implications: This novel immediate interaction between corticosteroids and cationic β2-agonist bronchodilators supports the use of such combinations in the pharmacotherapy of asthma.

Original languageEnglish (US)
Pages (from-to)1103-1109
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume120
Issue number5
DOIs
StatePublished - Nov 1 2007

Keywords

  • Bronchial smooth muscle
  • budesonide
  • corticosteroids
  • fluticasone
  • formoterol
  • long-acting β-agonists
  • nongenomic
  • organic cation transporter
  • salmeterol

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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