Abstract
Microtubule-associated protein 2 (MAP2) immunohistochemical labeling in the hippocampus was studied to assess the protective effect of brain perfusion during surgery requiring hypothermic circulatory arrest in 24 pigs exposed to anesthesia alone (control), 120 min of complete circulatory arrest at 15°C, 120 min of retrograde cerebral perfusion at 15°C, or 120 min of anterograde cerebral perfusion at 15°C. Pigs were reperfused for 60 min and sacrificed. In the control and anterograde perfusion groups, the intensity of MAP2 labeling was similar in all regions of the hippocampus. Circulatory arrest and retrograde perfusion resulted in significant reduction of MAP2 labeling (28% and 38% respectively of control, P < 0.001) of neurons in the CA1 region. MAP2 labeling may be useful for assessing early damage in the hippocampus in this model.
| Original language | English |
|---|---|
| Pages (from-to) | 9-12 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 222 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 24 1997 |
| Externally published | Yes |
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Keywords
- Brain ischemia
- Circulatory arrest
- Hippocampus
- Microtubule-associated protein 2
- Pig
- Retrograde perfusion
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
The effect of circulatory arrest and retrograde cerebral perfusion on microtubule-associated protein 2 : An immunohistochemical study in pig hippocampus. / Ye, Jian; Yang, Luojia; Del Bigio, Marc R.; Summers, Randy; Salerno, Tomas; Deslauriers, Roxanne.
In: Neuroscience Letters, Vol. 222, No. 1, 24.01.1997, p. 9-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effect of circulatory arrest and retrograde cerebral perfusion on microtubule-associated protein 2
T2 - An immunohistochemical study in pig hippocampus
AU - Ye, Jian
AU - Yang, Luojia
AU - Del Bigio, Marc R.
AU - Summers, Randy
AU - Salerno, Tomas
AU - Deslauriers, Roxanne
PY - 1997/1/24
Y1 - 1997/1/24
N2 - Microtubule-associated protein 2 (MAP2) immunohistochemical labeling in the hippocampus was studied to assess the protective effect of brain perfusion during surgery requiring hypothermic circulatory arrest in 24 pigs exposed to anesthesia alone (control), 120 min of complete circulatory arrest at 15°C, 120 min of retrograde cerebral perfusion at 15°C, or 120 min of anterograde cerebral perfusion at 15°C. Pigs were reperfused for 60 min and sacrificed. In the control and anterograde perfusion groups, the intensity of MAP2 labeling was similar in all regions of the hippocampus. Circulatory arrest and retrograde perfusion resulted in significant reduction of MAP2 labeling (28% and 38% respectively of control, P < 0.001) of neurons in the CA1 region. MAP2 labeling may be useful for assessing early damage in the hippocampus in this model.
AB - Microtubule-associated protein 2 (MAP2) immunohistochemical labeling in the hippocampus was studied to assess the protective effect of brain perfusion during surgery requiring hypothermic circulatory arrest in 24 pigs exposed to anesthesia alone (control), 120 min of complete circulatory arrest at 15°C, 120 min of retrograde cerebral perfusion at 15°C, or 120 min of anterograde cerebral perfusion at 15°C. Pigs were reperfused for 60 min and sacrificed. In the control and anterograde perfusion groups, the intensity of MAP2 labeling was similar in all regions of the hippocampus. Circulatory arrest and retrograde perfusion resulted in significant reduction of MAP2 labeling (28% and 38% respectively of control, P < 0.001) of neurons in the CA1 region. MAP2 labeling may be useful for assessing early damage in the hippocampus in this model.
KW - Brain ischemia
KW - Circulatory arrest
KW - Hippocampus
KW - Microtubule-associated protein 2
KW - Pig
KW - Retrograde perfusion
UR - http://www.scopus.com/inward/record.url?scp=0031019911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031019911&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(97)13328-6
DO - 10.1016/S0304-3940(97)13328-6
M3 - Article
C2 - 9121727
AN - SCOPUS:0031019911
VL - 222
SP - 9
EP - 12
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -