The effect of an antimicrobial gauze dressing impregnated with 0.2-percent polyhexamethylene biguanide as a barrier to prevent Pseudomonas aeruginosa wound invasion

Alejandro Cazzaniga, Victoria Serralta, Stephen Davis, Robert Orr, William Eaglstein, Patricia M. Mertz

Research output: Contribution to journalArticle

36 Scopus citations


An antimicrobial gauze has been developed that is a pre-washed, fluff-dried woven fabric with a crinkle weave pattern impregnated with 0.2-percent polyhexamethylene biguanide (PHMB) antimicrobial. The purpose of this study was to evaluate the effect of this dressing as a barrier for prevention of wound invasion by Pseudomonas aeruginosa (P. aeruginosa), a common wound pathogen. The microbial barrier study had two treatment groups: 1) gauze impregnated with PHMB and 2) gauze without an antimicrobial agent. The dressings covering the wounds were challenged with 100mL of 6.6 ± 0.3 log CFU/mL of P. aeruginosa. A secondary polyurethane film dressing was applied over all dressings to maintain a moist environment. Both wounds and dressings were analyzed for bacterial load at 24-, 48-, and 72-hours post-inoculation. The microbial barrier study showed that the PHMB dressing substantially reduced (or eliminated) by 4 to 5 logs the amount of P. aeruginosa that gained access to the wound bed at all assessment times. In addition, this dressing was able to reduce the inoculum within the dressing itself at 24- and 48-hours post-inoculation compared to control (p = 0.05). The normal flora (total bacteria) persisted in the wounded area as expected. In conclusion, this data suggests that in our swine model of partial-thickness wounds, the gauze dressing containing PHMB can effectively protect the wounds from outside invasion by P. aeruginosa bacteria.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
Issue number5
StatePublished - Dec 1 2002


ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

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